Imperfection and radiation damage in protein crystals studied with coherent radiation.
Fringes and speckles occur within diffraction spots when a crystal is illuminated with coherent radiation during X-ray diffraction. The additional information in these features provides insight into the imperfections in the crystal at the sub-micrometre scale. In addition, these features can provide...
| Autors principals: | , , , , , , |
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| Format: | Journal article |
| Idioma: | English |
| Publicat: |
International Union of Crystallography
2016
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| _version_ | 1826277287870332928 |
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| author | Nave, C Sutton, G Evans, G Owen, R Rau, C Robinson, I Stuart, D |
| author_facet | Nave, C Sutton, G Evans, G Owen, R Rau, C Robinson, I Stuart, D |
| author_sort | Nave, C |
| collection | OXFORD |
| description | Fringes and speckles occur within diffraction spots when a crystal is illuminated with coherent radiation during X-ray diffraction. The additional information in these features provides insight into the imperfections in the crystal at the sub-micrometre scale. In addition, these features can provide more accurate intensity measurements (e.g. by model-based profile fitting), detwinning (by distinguishing the various components), phasing (by exploiting sampling of the molecular transform) and refinement (by distinguishing regions with different unit-cell parameters). In order to exploit these potential benefits, the features due to coherent diffraction have to be recorded and any change due to radiation damage properly modelled. Initial results from recording coherent diffraction at cryotemperatures from polyhedrin crystals of approximately 2 µm in size are described. These measurements allowed information about the type of crystal imperfections to be obtained at the sub-micrometre level, together with the changes due to radiation damage. |
| first_indexed | 2024-03-06T23:26:38Z |
| format | Journal article |
| id | oxford-uuid:6a965bbf-225e-42a4-8a36-db1d3e982d8e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:26:38Z |
| publishDate | 2016 |
| publisher | International Union of Crystallography |
| record_format | dspace |
| spelling | oxford-uuid:6a965bbf-225e-42a4-8a36-db1d3e982d8e2022-03-26T18:58:27ZImperfection and radiation damage in protein crystals studied with coherent radiation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6a965bbf-225e-42a4-8a36-db1d3e982d8eEnglishSymplectic Elements at OxfordInternational Union of Crystallography2016Nave, CSutton, GEvans, GOwen, RRau, CRobinson, IStuart, DFringes and speckles occur within diffraction spots when a crystal is illuminated with coherent radiation during X-ray diffraction. The additional information in these features provides insight into the imperfections in the crystal at the sub-micrometre scale. In addition, these features can provide more accurate intensity measurements (e.g. by model-based profile fitting), detwinning (by distinguishing the various components), phasing (by exploiting sampling of the molecular transform) and refinement (by distinguishing regions with different unit-cell parameters). In order to exploit these potential benefits, the features due to coherent diffraction have to be recorded and any change due to radiation damage properly modelled. Initial results from recording coherent diffraction at cryotemperatures from polyhedrin crystals of approximately 2 µm in size are described. These measurements allowed information about the type of crystal imperfections to be obtained at the sub-micrometre level, together with the changes due to radiation damage. |
| spellingShingle | Nave, C Sutton, G Evans, G Owen, R Rau, C Robinson, I Stuart, D Imperfection and radiation damage in protein crystals studied with coherent radiation. |
| title | Imperfection and radiation damage in protein crystals studied with coherent radiation. |
| title_full | Imperfection and radiation damage in protein crystals studied with coherent radiation. |
| title_fullStr | Imperfection and radiation damage in protein crystals studied with coherent radiation. |
| title_full_unstemmed | Imperfection and radiation damage in protein crystals studied with coherent radiation. |
| title_short | Imperfection and radiation damage in protein crystals studied with coherent radiation. |
| title_sort | imperfection and radiation damage in protein crystals studied with coherent radiation |
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