TNFα signalling primes chromatin for NF-κB binding and induces rapid and widespread nucleosome repositioning.
The rearrangement of nucleosomes along the DNA fiber profoundly affects gene expression, but little is known about how signalling reshapes the chromatin landscape, in three-dimensional space and over time, to allow establishment of new transcriptional programs.Using micrococcal nuclease treatment an...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2014
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| _version_ | 1797073872538828800 |
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| author | Diermeier, S Kolovos, P Heizinger, L Schwartz, U Georgomanolis, T Zirkel, A Wedemann, G Grosveld, F Knoch, T Merkl, R Cook, P Längst, G Papantonis, A |
| author_facet | Diermeier, S Kolovos, P Heizinger, L Schwartz, U Georgomanolis, T Zirkel, A Wedemann, G Grosveld, F Knoch, T Merkl, R Cook, P Längst, G Papantonis, A |
| author_sort | Diermeier, S |
| collection | OXFORD |
| description | The rearrangement of nucleosomes along the DNA fiber profoundly affects gene expression, but little is known about how signalling reshapes the chromatin landscape, in three-dimensional space and over time, to allow establishment of new transcriptional programs.Using micrococcal nuclease treatment and high-throughput sequencing, we map genome-wide changes in nucleosome positioning in primary human endothelial cells stimulated with tumour necrosis factor alpha (TNFα) - a proinflammatory cytokine that signals through nuclear factor kappa-B (NF-κB). Within 10 min, nucleosomes reposition at regions both proximal and distal to NF-κB binding sites, before the transcription factor quantitatively binds thereon. Similarly, in long TNFα-responsive genes, repositioning precedes transcription by pioneering elongating polymerases and appears to nucleate from intragenic enhancer clusters resembling super-enhancers. By 30 min, widespread repositioning throughout megabase pair-long chromosomal segments, with consequential effects on three-dimensional structure (detected using chromosome conformation capture), is seen.Whilst nucleosome repositioning is viewed as a local phenomenon, our results point to effects occurring over multiple scales. Here, we present data in support of a TNFα-induced priming mechanism, mostly independent of NF-κB binding and/or elongating RNA polymerases, leading to a plastic network of interactions that affects DNA accessibility over large domains. |
| first_indexed | 2024-03-06T23:28:11Z |
| format | Journal article |
| id | oxford-uuid:6b1e02ed-db4f-43fc-aa68-44e880e81dab |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:28:11Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | oxford-uuid:6b1e02ed-db4f-43fc-aa68-44e880e81dab2022-03-26T19:01:47ZTNFα signalling primes chromatin for NF-κB binding and induces rapid and widespread nucleosome repositioning.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6b1e02ed-db4f-43fc-aa68-44e880e81dabEnglishSymplectic Elements at Oxford2014Diermeier, SKolovos, PHeizinger, LSchwartz, UGeorgomanolis, TZirkel, AWedemann, GGrosveld, FKnoch, TMerkl, RCook, PLängst, GPapantonis, AThe rearrangement of nucleosomes along the DNA fiber profoundly affects gene expression, but little is known about how signalling reshapes the chromatin landscape, in three-dimensional space and over time, to allow establishment of new transcriptional programs.Using micrococcal nuclease treatment and high-throughput sequencing, we map genome-wide changes in nucleosome positioning in primary human endothelial cells stimulated with tumour necrosis factor alpha (TNFα) - a proinflammatory cytokine that signals through nuclear factor kappa-B (NF-κB). Within 10 min, nucleosomes reposition at regions both proximal and distal to NF-κB binding sites, before the transcription factor quantitatively binds thereon. Similarly, in long TNFα-responsive genes, repositioning precedes transcription by pioneering elongating polymerases and appears to nucleate from intragenic enhancer clusters resembling super-enhancers. By 30 min, widespread repositioning throughout megabase pair-long chromosomal segments, with consequential effects on three-dimensional structure (detected using chromosome conformation capture), is seen.Whilst nucleosome repositioning is viewed as a local phenomenon, our results point to effects occurring over multiple scales. Here, we present data in support of a TNFα-induced priming mechanism, mostly independent of NF-κB binding and/or elongating RNA polymerases, leading to a plastic network of interactions that affects DNA accessibility over large domains. |
| spellingShingle | Diermeier, S Kolovos, P Heizinger, L Schwartz, U Georgomanolis, T Zirkel, A Wedemann, G Grosveld, F Knoch, T Merkl, R Cook, P Längst, G Papantonis, A TNFα signalling primes chromatin for NF-κB binding and induces rapid and widespread nucleosome repositioning. |
| title | TNFα signalling primes chromatin for NF-κB binding and induces rapid and widespread nucleosome repositioning. |
| title_full | TNFα signalling primes chromatin for NF-κB binding and induces rapid and widespread nucleosome repositioning. |
| title_fullStr | TNFα signalling primes chromatin for NF-κB binding and induces rapid and widespread nucleosome repositioning. |
| title_full_unstemmed | TNFα signalling primes chromatin for NF-κB binding and induces rapid and widespread nucleosome repositioning. |
| title_short | TNFα signalling primes chromatin for NF-κB binding and induces rapid and widespread nucleosome repositioning. |
| title_sort | tnfα signalling primes chromatin for nf κb binding and induces rapid and widespread nucleosome repositioning |
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