High-throughput phenotyping reveals expansive genetic and structural underpinnings of immune variation
By developing a high-density murine immunophenotyping platform compatible with highthroughput genetic screening, we have established profound contributions of genetics and structure to immune variation (www.immunophenotype.org). Specifically, highthroughput phenotyping of 530 unique mouse gene knock...
| Հիմնական հեղինակներ: | , , , , , , , , , , , , |
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| Ձևաչափ: | Journal article |
| Հրապարակվել է: |
Springer Nature
2019
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| _version_ | 1826277468800024576 |
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| author | Abeler-Dörner, L Laing, A Lorenc, A Bull, K Crockford, T Cawthorne, E Edmans, M Cornall, R Morón, B Song-Zhao, G Abeler, J Powrie, F Et al. |
| author_facet | Abeler-Dörner, L Laing, A Lorenc, A Bull, K Crockford, T Cawthorne, E Edmans, M Cornall, R Morón, B Song-Zhao, G Abeler, J Powrie, F Et al. |
| author_sort | Abeler-Dörner, L |
| collection | OXFORD |
| description | By developing a high-density murine immunophenotyping platform compatible with highthroughput genetic screening, we have established profound contributions of genetics and structure to immune variation (www.immunophenotype.org). Specifically, highthroughput phenotyping of 530 unique mouse gene knockouts identified 140 monogenic “hits”, of which most had no previous immunological association. Furthermore, hits were collectively enriched in genes for which humans show poor tolerance to loss-of-function. The immunophenotyping platform also exposed dense correlation networks linking immune parameters with one another and with specific physiologic traits. Such linkages limit freedom-of-movement for individual immune parameters, thereby imposing genetically regulated “immunological structures”, whose integrity was associated with immunocompetence. Hence, we provide an expanded genetic resource and structural perspective for understanding and monitoring immune variation in health and disease. |
| first_indexed | 2024-03-06T23:29:21Z |
| format | Journal article |
| id | oxford-uuid:6b7b22c5-d43b-4422-bb82-c3fb5fc9c7f4 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T23:29:21Z |
| publishDate | 2019 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | oxford-uuid:6b7b22c5-d43b-4422-bb82-c3fb5fc9c7f42022-03-26T19:04:25ZHigh-throughput phenotyping reveals expansive genetic and structural underpinnings of immune variationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6b7b22c5-d43b-4422-bb82-c3fb5fc9c7f4Symplectic Elements at OxfordSpringer Nature2019Abeler-Dörner, LLaing, ALorenc, ABull, KCrockford, TCawthorne, EEdmans, MCornall, RMorón, BSong-Zhao, GAbeler, JPowrie, FEt al.By developing a high-density murine immunophenotyping platform compatible with highthroughput genetic screening, we have established profound contributions of genetics and structure to immune variation (www.immunophenotype.org). Specifically, highthroughput phenotyping of 530 unique mouse gene knockouts identified 140 monogenic “hits”, of which most had no previous immunological association. Furthermore, hits were collectively enriched in genes for which humans show poor tolerance to loss-of-function. The immunophenotyping platform also exposed dense correlation networks linking immune parameters with one another and with specific physiologic traits. Such linkages limit freedom-of-movement for individual immune parameters, thereby imposing genetically regulated “immunological structures”, whose integrity was associated with immunocompetence. Hence, we provide an expanded genetic resource and structural perspective for understanding and monitoring immune variation in health and disease. |
| spellingShingle | Abeler-Dörner, L Laing, A Lorenc, A Bull, K Crockford, T Cawthorne, E Edmans, M Cornall, R Morón, B Song-Zhao, G Abeler, J Powrie, F Et al. High-throughput phenotyping reveals expansive genetic and structural underpinnings of immune variation |
| title | High-throughput phenotyping reveals expansive genetic and structural underpinnings of immune variation |
| title_full | High-throughput phenotyping reveals expansive genetic and structural underpinnings of immune variation |
| title_fullStr | High-throughput phenotyping reveals expansive genetic and structural underpinnings of immune variation |
| title_full_unstemmed | High-throughput phenotyping reveals expansive genetic and structural underpinnings of immune variation |
| title_short | High-throughput phenotyping reveals expansive genetic and structural underpinnings of immune variation |
| title_sort | high throughput phenotyping reveals expansive genetic and structural underpinnings of immune variation |
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