Adhesion molecule expression on vascular endothelium and nitroblue tetrazolium reducing activity in human colonic mucosa.
BACKGROUND: Expression of adhesion molecules is increased in inflamed colonic mucosa, but little is known about their functional activity in vascular endothelium. METHODS: We studied in situ nitroblue tetrazolium reducing activity and expression of E-selectin, ICAM-1, CD31, and VCAM-1 by immunohisto...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1995
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| _version_ | 1797074059428626432 |
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| author | Oshitani, N Campbell, A Bloom, S Kitano, A Kobayashi, K Jewell, D |
| author_facet | Oshitani, N Campbell, A Bloom, S Kitano, A Kobayashi, K Jewell, D |
| author_sort | Oshitani, N |
| collection | OXFORD |
| description | BACKGROUND: Expression of adhesion molecules is increased in inflamed colonic mucosa, but little is known about their functional activity in vascular endothelium. METHODS: We studied in situ nitroblue tetrazolium reducing activity and expression of E-selectin, ICAM-1, CD31, and VCAM-1 by immunohistochemistry in the same biopsy specimen in controls and patients with ulcerative colitis (UC). RESULTS: VCAM-1 expression was negative in mucosal vessels. E-selectin-positive vessels were significantly increased in endoscopically active colitis compared with normal mucosa. ICAM-1-positive vessels were consistently found in normal, quiescent UC and active UC. CD31-positive vessels were not significantly increased in quiescent UC and active UC compared with control. Only E-selectin significantly correlated with the histologic grade of inflammation. Nitroblue tetrazolium reducing vessels were increased in inflamed mucosa, and these vessels expressed ICAM-1 and CD31. E-selectin positivity in association with nitroblue tetrazolium reduction was mainly seen in the large mucosal vessels, but capillaries showing nitroblue tetrazolium reduction were rarely positive for E-selectin. CONCLUSIONS: Phenotypic and functional activation of vascular endothelium might be involved in the recruitment of leukocytes and tissue destruction of inflamed colonic mucosa. |
| first_indexed | 2024-03-06T23:30:50Z |
| format | Journal article |
| id | oxford-uuid:6bf601f2-8a43-49be-9db6-d2d944c87694 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:30:50Z |
| publishDate | 1995 |
| record_format | dspace |
| spelling | oxford-uuid:6bf601f2-8a43-49be-9db6-d2d944c876942022-03-26T19:07:42ZAdhesion molecule expression on vascular endothelium and nitroblue tetrazolium reducing activity in human colonic mucosa.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6bf601f2-8a43-49be-9db6-d2d944c87694EnglishSymplectic Elements at Oxford1995Oshitani, NCampbell, ABloom, SKitano, AKobayashi, KJewell, DBACKGROUND: Expression of adhesion molecules is increased in inflamed colonic mucosa, but little is known about their functional activity in vascular endothelium. METHODS: We studied in situ nitroblue tetrazolium reducing activity and expression of E-selectin, ICAM-1, CD31, and VCAM-1 by immunohistochemistry in the same biopsy specimen in controls and patients with ulcerative colitis (UC). RESULTS: VCAM-1 expression was negative in mucosal vessels. E-selectin-positive vessels were significantly increased in endoscopically active colitis compared with normal mucosa. ICAM-1-positive vessels were consistently found in normal, quiescent UC and active UC. CD31-positive vessels were not significantly increased in quiescent UC and active UC compared with control. Only E-selectin significantly correlated with the histologic grade of inflammation. Nitroblue tetrazolium reducing vessels were increased in inflamed mucosa, and these vessels expressed ICAM-1 and CD31. E-selectin positivity in association with nitroblue tetrazolium reduction was mainly seen in the large mucosal vessels, but capillaries showing nitroblue tetrazolium reduction were rarely positive for E-selectin. CONCLUSIONS: Phenotypic and functional activation of vascular endothelium might be involved in the recruitment of leukocytes and tissue destruction of inflamed colonic mucosa. |
| spellingShingle | Oshitani, N Campbell, A Bloom, S Kitano, A Kobayashi, K Jewell, D Adhesion molecule expression on vascular endothelium and nitroblue tetrazolium reducing activity in human colonic mucosa. |
| title | Adhesion molecule expression on vascular endothelium and nitroblue tetrazolium reducing activity in human colonic mucosa. |
| title_full | Adhesion molecule expression on vascular endothelium and nitroblue tetrazolium reducing activity in human colonic mucosa. |
| title_fullStr | Adhesion molecule expression on vascular endothelium and nitroblue tetrazolium reducing activity in human colonic mucosa. |
| title_full_unstemmed | Adhesion molecule expression on vascular endothelium and nitroblue tetrazolium reducing activity in human colonic mucosa. |
| title_short | Adhesion molecule expression on vascular endothelium and nitroblue tetrazolium reducing activity in human colonic mucosa. |
| title_sort | adhesion molecule expression on vascular endothelium and nitroblue tetrazolium reducing activity in human colonic mucosa |
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