Organelle motility in plant cells: imaging golgi and ER dynamics with GFP.
By the addition of plant or mammalian targeting sequences, green fluorescent protein (GFP) can be directed to the endoplasmic reticulum, the Golgi apparatus, or both organelles in plant cells. This unit describes the application of rapid Agrobacterium and virus-mediated transient expression systems...
Main Authors: | , , , |
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格式: | Journal article |
語言: | English |
出版: |
2001
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_version_ | 1826277587542867968 |
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author | Hawes, C Brandizzil, F Batoko, H Moore, I |
author_facet | Hawes, C Brandizzil, F Batoko, H Moore, I |
author_sort | Hawes, C |
collection | OXFORD |
description | By the addition of plant or mammalian targeting sequences, green fluorescent protein (GFP) can be directed to the endoplasmic reticulum, the Golgi apparatus, or both organelles in plant cells. This unit describes the application of rapid Agrobacterium and virus-mediated transient expression systems in leaf tissue to permit visualization of ER and Golgi dynamics in vivo by epifluorescence or confocal microscopy. |
first_indexed | 2024-03-06T23:31:09Z |
format | Journal article |
id | oxford-uuid:6c108047-ebd4-4493-b542-c6521f3b2ca1 |
institution | University of Oxford |
language | English |
last_indexed | 2024-03-06T23:31:09Z |
publishDate | 2001 |
record_format | dspace |
spelling | oxford-uuid:6c108047-ebd4-4493-b542-c6521f3b2ca12022-03-26T19:08:21ZOrganelle motility in plant cells: imaging golgi and ER dynamics with GFP.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6c108047-ebd4-4493-b542-c6521f3b2ca1EnglishSymplectic Elements at Oxford2001Hawes, CBrandizzil, FBatoko, HMoore, IBy the addition of plant or mammalian targeting sequences, green fluorescent protein (GFP) can be directed to the endoplasmic reticulum, the Golgi apparatus, or both organelles in plant cells. This unit describes the application of rapid Agrobacterium and virus-mediated transient expression systems in leaf tissue to permit visualization of ER and Golgi dynamics in vivo by epifluorescence or confocal microscopy. |
spellingShingle | Hawes, C Brandizzil, F Batoko, H Moore, I Organelle motility in plant cells: imaging golgi and ER dynamics with GFP. |
title | Organelle motility in plant cells: imaging golgi and ER dynamics with GFP. |
title_full | Organelle motility in plant cells: imaging golgi and ER dynamics with GFP. |
title_fullStr | Organelle motility in plant cells: imaging golgi and ER dynamics with GFP. |
title_full_unstemmed | Organelle motility in plant cells: imaging golgi and ER dynamics with GFP. |
title_short | Organelle motility in plant cells: imaging golgi and ER dynamics with GFP. |
title_sort | organelle motility in plant cells imaging golgi and er dynamics with gfp |
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