Arginine methylation controls growth regulation by E2F-1

E2F transcription factors are implicated in diverse cellular functions. The founding member, E2F-1, is endowed with contradictory activities, being able to promote cell-cycle progression and induce apoptosis. However, the mechanisms that underlie the opposing outcomes of E2F-1 activation remain larg...

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Main Authors: Cho, E, Zheng, S, Munro, S, Liu, G, Carr, S, Moehlenbrink, J, Lu, Y, Stimson, L, Khan, O, Konietzny, R, McGouran, J, Coutts, A, Kessler, B, Kerr, D, Thangue, N
Format: Journal article
Language:English
Published: 2012
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author Cho, E
Zheng, S
Munro, S
Liu, G
Carr, S
Moehlenbrink, J
Lu, Y
Stimson, L
Khan, O
Konietzny, R
McGouran, J
Coutts, A
Kessler, B
Kerr, D
Thangue, N
author_facet Cho, E
Zheng, S
Munro, S
Liu, G
Carr, S
Moehlenbrink, J
Lu, Y
Stimson, L
Khan, O
Konietzny, R
McGouran, J
Coutts, A
Kessler, B
Kerr, D
Thangue, N
author_sort Cho, E
collection OXFORD
description E2F transcription factors are implicated in diverse cellular functions. The founding member, E2F-1, is endowed with contradictory activities, being able to promote cell-cycle progression and induce apoptosis. However, the mechanisms that underlie the opposing outcomes of E2F-1 activation remain largely unknown. We show here that E2F-1 is directly methylated by PRMT5 (protein arginine methyltransferase 5), and that arginine methylation is responsible for regulating its biochemical and functional properties, which impacts on E2F-1-dependent growth control. Thus, depleting PRMT5 causes increased E2F-1 protein levels, which coincides with decreased growth rate and associated apoptosis. Arginine methylation influences E2F-1 protein stability, and the enhanced transcription of a variety of downstream target genes reflects increased E2F-1 DNA-binding activity. Importantly, E2F-1 is methylated in tumour cells, and a reduced level of methylation is evident under DNA damage conditions that allow E2F-1 stabilization and give rise to apoptosis. Significantly, in a subgroup of colorectal cancer, high levels of PRMT5 frequently coincide with low levels of E2F-1 and reflect a poor clinical outcome. Our results establish that arginine methylation regulates the biological activity of E2F-1 activity, and raise the possibility that arginine methylation contributes to tumourigenesis by influencing the E2F pathway. © 2012 European Molecular Biology Organization | All Rights Reserved.
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spelling oxford-uuid:6c7cba23-0236-45ff-8877-4f895f8fb2f02022-03-26T19:11:11ZArginine methylation controls growth regulation by E2F-1Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6c7cba23-0236-45ff-8877-4f895f8fb2f0EnglishSymplectic Elements at Oxford2012Cho, EZheng, SMunro, SLiu, GCarr, SMoehlenbrink, JLu, YStimson, LKhan, OKonietzny, RMcGouran, JCoutts, AKessler, BKerr, DThangue, NE2F transcription factors are implicated in diverse cellular functions. The founding member, E2F-1, is endowed with contradictory activities, being able to promote cell-cycle progression and induce apoptosis. However, the mechanisms that underlie the opposing outcomes of E2F-1 activation remain largely unknown. We show here that E2F-1 is directly methylated by PRMT5 (protein arginine methyltransferase 5), and that arginine methylation is responsible for regulating its biochemical and functional properties, which impacts on E2F-1-dependent growth control. Thus, depleting PRMT5 causes increased E2F-1 protein levels, which coincides with decreased growth rate and associated apoptosis. Arginine methylation influences E2F-1 protein stability, and the enhanced transcription of a variety of downstream target genes reflects increased E2F-1 DNA-binding activity. Importantly, E2F-1 is methylated in tumour cells, and a reduced level of methylation is evident under DNA damage conditions that allow E2F-1 stabilization and give rise to apoptosis. Significantly, in a subgroup of colorectal cancer, high levels of PRMT5 frequently coincide with low levels of E2F-1 and reflect a poor clinical outcome. Our results establish that arginine methylation regulates the biological activity of E2F-1 activity, and raise the possibility that arginine methylation contributes to tumourigenesis by influencing the E2F pathway. © 2012 European Molecular Biology Organization | All Rights Reserved.
spellingShingle Cho, E
Zheng, S
Munro, S
Liu, G
Carr, S
Moehlenbrink, J
Lu, Y
Stimson, L
Khan, O
Konietzny, R
McGouran, J
Coutts, A
Kessler, B
Kerr, D
Thangue, N
Arginine methylation controls growth regulation by E2F-1
title Arginine methylation controls growth regulation by E2F-1
title_full Arginine methylation controls growth regulation by E2F-1
title_fullStr Arginine methylation controls growth regulation by E2F-1
title_full_unstemmed Arginine methylation controls growth regulation by E2F-1
title_short Arginine methylation controls growth regulation by E2F-1
title_sort arginine methylation controls growth regulation by e2f 1
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