Characterization of the oligosaccharide component of microsomal beta-glucuronidase from rat liver.
The oligosaccharides of microsomal beta-glucuronidase were analysed by gel permeation and weak anion exchange chromatography following hydrazine release. N-linked glycans, constituted 80% of the total glycan pool and were mainly of the tri- and biantennary complex type with or without core and arm f...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2004
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| _version_ | 1826277715117867008 |
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| author | Hoja-Łukowicz, D Butters, T Lityńska, A |
| author_facet | Hoja-Łukowicz, D Butters, T Lityńska, A |
| author_sort | Hoja-Łukowicz, D |
| collection | OXFORD |
| description | The oligosaccharides of microsomal beta-glucuronidase were analysed by gel permeation and weak anion exchange chromatography following hydrazine release. N-linked glycans, constituted 80% of the total glycan pool and were mainly of the tri- and biantennary complex type with or without core and arm fucose. The major oligosaccharide, that comprised 30.6% of all the species analysed, was structurally identified by reagent array analysis method and found to be a triantennary complex structure, Galbeta1,4GlcNAcbeta1,2Manalpha1,6(3)(Galbeta1,4GlcNAcbeta1,4(Galbeta1,4GlcNAcbeta1,2) Manalpha1,3(6))Manbeta1,4GlcNAcbeta1,4 GlcNAc. O-Linked glycans comprised 20% of the total glycan pool, the major species being Galbeta1,3GalNAc. All of the N- and O-linked glycans were charged. Most of the negative charge was due to sialic acid (85.0%) with the remainder being phosphate present as phosphomonoesters (7.3%) and phosphodiesters (5%). This is the first report of O-linked carbohydrate chains in microsomal beta-glucuronidase. The presence of O-linked glycans and branched N-linked glycans in a microsomal enzyme, in relation to the current view of glycosyltransferase compartmentalization in the Golgi is discussed. |
| first_indexed | 2024-03-06T23:33:05Z |
| format | Journal article |
| id | oxford-uuid:6cbaa73f-4dee-49bd-b851-4347bd12f406 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:33:05Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:6cbaa73f-4dee-49bd-b851-4347bd12f4062022-03-26T19:12:57ZCharacterization of the oligosaccharide component of microsomal beta-glucuronidase from rat liver.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6cbaa73f-4dee-49bd-b851-4347bd12f406EnglishSymplectic Elements at Oxford2004Hoja-Łukowicz, DButters, TLityńska, AThe oligosaccharides of microsomal beta-glucuronidase were analysed by gel permeation and weak anion exchange chromatography following hydrazine release. N-linked glycans, constituted 80% of the total glycan pool and were mainly of the tri- and biantennary complex type with or without core and arm fucose. The major oligosaccharide, that comprised 30.6% of all the species analysed, was structurally identified by reagent array analysis method and found to be a triantennary complex structure, Galbeta1,4GlcNAcbeta1,2Manalpha1,6(3)(Galbeta1,4GlcNAcbeta1,4(Galbeta1,4GlcNAcbeta1,2) Manalpha1,3(6))Manbeta1,4GlcNAcbeta1,4 GlcNAc. O-Linked glycans comprised 20% of the total glycan pool, the major species being Galbeta1,3GalNAc. All of the N- and O-linked glycans were charged. Most of the negative charge was due to sialic acid (85.0%) with the remainder being phosphate present as phosphomonoesters (7.3%) and phosphodiesters (5%). This is the first report of O-linked carbohydrate chains in microsomal beta-glucuronidase. The presence of O-linked glycans and branched N-linked glycans in a microsomal enzyme, in relation to the current view of glycosyltransferase compartmentalization in the Golgi is discussed. |
| spellingShingle | Hoja-Łukowicz, D Butters, T Lityńska, A Characterization of the oligosaccharide component of microsomal beta-glucuronidase from rat liver. |
| title | Characterization of the oligosaccharide component of microsomal beta-glucuronidase from rat liver. |
| title_full | Characterization of the oligosaccharide component of microsomal beta-glucuronidase from rat liver. |
| title_fullStr | Characterization of the oligosaccharide component of microsomal beta-glucuronidase from rat liver. |
| title_full_unstemmed | Characterization of the oligosaccharide component of microsomal beta-glucuronidase from rat liver. |
| title_short | Characterization of the oligosaccharide component of microsomal beta-glucuronidase from rat liver. |
| title_sort | characterization of the oligosaccharide component of microsomal beta glucuronidase from rat liver |
| work_keys_str_mv | AT hojałukowiczd characterizationoftheoligosaccharidecomponentofmicrosomalbetaglucuronidasefromratliver AT butterst characterizationoftheoligosaccharidecomponentofmicrosomalbetaglucuronidasefromratliver AT litynskaa characterizationoftheoligosaccharidecomponentofmicrosomalbetaglucuronidasefromratliver |