Antiviral immune responses of mice lacking MHC class II or its associated invariant chain.
Induction of T-helper cells and T-B cell interaction have been considered to critically depend upon recognition of major histocompatibility complex (MHC) class II molecules by the T cell receptor. Mice lacking either MHC class II molecules (class II(0/0) mice) or its associated invariant chain (Ii0/...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Sprog: | English |
| Udgivet: |
1996
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| _version_ | 1826277816910479360 |
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| author | Battegay, M Bachmann, M Burhkart, C Viville, S Benoist, C Mathis, D Hengartner, H Zinkernagel, R |
| author_facet | Battegay, M Bachmann, M Burhkart, C Viville, S Benoist, C Mathis, D Hengartner, H Zinkernagel, R |
| author_sort | Battegay, M |
| collection | OXFORD |
| description | Induction of T-helper cells and T-B cell interaction have been considered to critically depend upon recognition of major histocompatibility complex (MHC) class II molecules by the T cell receptor. Mice lacking either MHC class II molecules (class II(0/0) mice) or its associated invariant chain (Ii0/0 mice) provide new opportunities to test this premise. Immune responses to some protein antigens have been studied in these mice; little is known about their ability to withstand viral infections. We therefore tested CD8+ effector T cells and CD4+ T-cell-dependent B cell function during different viral infections. The vesicular stomatitis virus (VSV)-specific primary cytotoxic T cell response which is largely T-helper-dependent was diminished in Ii(0/0) and absent in class II(0/0) mice. The usually less T-helper-dependent cytotoxic vaccinia or lymphocytic choriomeningitis virus (LCMV)-specific CD8+ T cell responses were reduced up to ninefold in class II(0/0) and up to threefold in Ii(0/0) mice. In class II(0/0) mice, the T-helper-independent neutralizing IgM response against the glycoprotein of VSV was within normal ranges but, in contrast to previous results on CD4(0/0) mice, the T-helper-dependent IgG response was absent. Ii(0/0) mice exhibited a normal neutralizing IgM response; in contrast to class II(0/0) mice, they mounted a significant, though reduced specific IgG response. Similar results were obtained for antibody responses against the nucleoprotein of VSV. Although the T-helper-cell response upon infection with VSV seemed diminished only a little in Ii(0/0) mice, presentation of VSV-G to a class II-restricted specific hybridoma was greater than 300-fold reduced in the absence of Ii. This suggests that local protein concentrations reached during viral infection in the host are high enough to override the Ii deficiency of antigen-presenting cells in vivo. |
| first_indexed | 2024-03-06T23:34:36Z |
| format | Journal article |
| id | oxford-uuid:6d37fe7f-1e7a-46b6-b18e-f562fd8989df |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:34:36Z |
| publishDate | 1996 |
| record_format | dspace |
| spelling | oxford-uuid:6d37fe7f-1e7a-46b6-b18e-f562fd8989df2022-03-26T19:16:26ZAntiviral immune responses of mice lacking MHC class II or its associated invariant chain.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6d37fe7f-1e7a-46b6-b18e-f562fd8989dfEnglishSymplectic Elements at Oxford1996Battegay, MBachmann, MBurhkart, CViville, SBenoist, CMathis, DHengartner, HZinkernagel, RInduction of T-helper cells and T-B cell interaction have been considered to critically depend upon recognition of major histocompatibility complex (MHC) class II molecules by the T cell receptor. Mice lacking either MHC class II molecules (class II(0/0) mice) or its associated invariant chain (Ii0/0 mice) provide new opportunities to test this premise. Immune responses to some protein antigens have been studied in these mice; little is known about their ability to withstand viral infections. We therefore tested CD8+ effector T cells and CD4+ T-cell-dependent B cell function during different viral infections. The vesicular stomatitis virus (VSV)-specific primary cytotoxic T cell response which is largely T-helper-dependent was diminished in Ii(0/0) and absent in class II(0/0) mice. The usually less T-helper-dependent cytotoxic vaccinia or lymphocytic choriomeningitis virus (LCMV)-specific CD8+ T cell responses were reduced up to ninefold in class II(0/0) and up to threefold in Ii(0/0) mice. In class II(0/0) mice, the T-helper-independent neutralizing IgM response against the glycoprotein of VSV was within normal ranges but, in contrast to previous results on CD4(0/0) mice, the T-helper-dependent IgG response was absent. Ii(0/0) mice exhibited a normal neutralizing IgM response; in contrast to class II(0/0) mice, they mounted a significant, though reduced specific IgG response. Similar results were obtained for antibody responses against the nucleoprotein of VSV. Although the T-helper-cell response upon infection with VSV seemed diminished only a little in Ii(0/0) mice, presentation of VSV-G to a class II-restricted specific hybridoma was greater than 300-fold reduced in the absence of Ii. This suggests that local protein concentrations reached during viral infection in the host are high enough to override the Ii deficiency of antigen-presenting cells in vivo. |
| spellingShingle | Battegay, M Bachmann, M Burhkart, C Viville, S Benoist, C Mathis, D Hengartner, H Zinkernagel, R Antiviral immune responses of mice lacking MHC class II or its associated invariant chain. |
| title | Antiviral immune responses of mice lacking MHC class II or its associated invariant chain. |
| title_full | Antiviral immune responses of mice lacking MHC class II or its associated invariant chain. |
| title_fullStr | Antiviral immune responses of mice lacking MHC class II or its associated invariant chain. |
| title_full_unstemmed | Antiviral immune responses of mice lacking MHC class II or its associated invariant chain. |
| title_short | Antiviral immune responses of mice lacking MHC class II or its associated invariant chain. |
| title_sort | antiviral immune responses of mice lacking mhc class ii or its associated invariant chain |
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