A dynamic coordination of flagellum and cytoplasmic cytoskeleton assembly specifies cell morphogenesis in trypanosomes.
Plasma membrane-to-plasma membrane connections are common features of eukaryotic cells, with cytoskeletal frameworks below the respective membranes underpinning these connections. A defining feature of Trypanosoma bruceiis the lateral attachment of its single flagellum to the cell body, which is med...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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Company of Biologists
2015
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| _version_ | 1797074389849604096 |
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| author | Sunter, J Varga, V Dean, S Gull, K |
| author_facet | Sunter, J Varga, V Dean, S Gull, K |
| author_sort | Sunter, J |
| collection | OXFORD |
| description | Plasma membrane-to-plasma membrane connections are common features of eukaryotic cells, with cytoskeletal frameworks below the respective membranes underpinning these connections. A defining feature of Trypanosoma bruceiis the lateral attachment of its single flagellum to the cell body, which is mediated by a cytoskeletal structure called the flagellum attachment zone (FAZ). The FAZ is a key morphogenetic structure. Disruption of FAZ assembly can lead to flagellum detachment and dramatic changes in cell shape. To understand this complex structure, the identity of more of its constituent proteins is required. Here, we have used both proteomics and bioinformatics to identify eight new FAZ proteins. Using inducible expression of FAZ proteins tagged with eYFP we demonstrate that the site of FAZ assembly is close to the flagellar pocket at the proximal end of the FAZ. This contrasts with the flagellum, which is assembled at its distal end; hence, these two interconnected cytoskeletal structures have distinct spatially separated assembly sites. This challenging result has many implications for understanding the process of cell morphogenesis and interpreting mutant phenotypes. |
| first_indexed | 2024-03-06T23:35:26Z |
| format | Journal article |
| id | oxford-uuid:6d7f1971-33c6-4cce-b0ca-0322f5809155 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:35:26Z |
| publishDate | 2015 |
| publisher | Company of Biologists |
| record_format | dspace |
| spelling | oxford-uuid:6d7f1971-33c6-4cce-b0ca-0322f58091552022-03-26T19:18:09ZA dynamic coordination of flagellum and cytoplasmic cytoskeleton assembly specifies cell morphogenesis in trypanosomes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6d7f1971-33c6-4cce-b0ca-0322f5809155EnglishSymplectic Elements at OxfordCompany of Biologists2015Sunter, JVarga, VDean, SGull, KPlasma membrane-to-plasma membrane connections are common features of eukaryotic cells, with cytoskeletal frameworks below the respective membranes underpinning these connections. A defining feature of Trypanosoma bruceiis the lateral attachment of its single flagellum to the cell body, which is mediated by a cytoskeletal structure called the flagellum attachment zone (FAZ). The FAZ is a key morphogenetic structure. Disruption of FAZ assembly can lead to flagellum detachment and dramatic changes in cell shape. To understand this complex structure, the identity of more of its constituent proteins is required. Here, we have used both proteomics and bioinformatics to identify eight new FAZ proteins. Using inducible expression of FAZ proteins tagged with eYFP we demonstrate that the site of FAZ assembly is close to the flagellar pocket at the proximal end of the FAZ. This contrasts with the flagellum, which is assembled at its distal end; hence, these two interconnected cytoskeletal structures have distinct spatially separated assembly sites. This challenging result has many implications for understanding the process of cell morphogenesis and interpreting mutant phenotypes. |
| spellingShingle | Sunter, J Varga, V Dean, S Gull, K A dynamic coordination of flagellum and cytoplasmic cytoskeleton assembly specifies cell morphogenesis in trypanosomes. |
| title | A dynamic coordination of flagellum and cytoplasmic cytoskeleton assembly specifies cell morphogenesis in trypanosomes. |
| title_full | A dynamic coordination of flagellum and cytoplasmic cytoskeleton assembly specifies cell morphogenesis in trypanosomes. |
| title_fullStr | A dynamic coordination of flagellum and cytoplasmic cytoskeleton assembly specifies cell morphogenesis in trypanosomes. |
| title_full_unstemmed | A dynamic coordination of flagellum and cytoplasmic cytoskeleton assembly specifies cell morphogenesis in trypanosomes. |
| title_short | A dynamic coordination of flagellum and cytoplasmic cytoskeleton assembly specifies cell morphogenesis in trypanosomes. |
| title_sort | dynamic coordination of flagellum and cytoplasmic cytoskeleton assembly specifies cell morphogenesis in trypanosomes |
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