Rapid ovarian transcript changes during the onset of premature ovarian insufficiency
Premature ovarian insufficiency (POI) affects 1-3% of women under 40 years of age. The identified causes are highly heterogeneous, and 70% of the cases are idiopathic. The ovarian manifestation varies from a variable population of follicles that fail to develop (follicular POI) to the absence of fol...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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Bioscientifica
2022
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| _version_ | 1797109957970100224 |
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| author | Kaune, H Montiel, JF Fenwick, M Williams, SA |
| author_facet | Kaune, H Montiel, JF Fenwick, M Williams, SA |
| author_sort | Kaune, H |
| collection | OXFORD |
| description | Premature ovarian insufficiency (POI) affects 1-3% of women under 40 years of age. The identified causes are highly heterogeneous, and 70% of the cases are idiopathic. The ovarian manifestation varies from a variable population of follicles that fail to develop (follicular POI) to the absence of follicles (afollicular POI) with a transition from one to the other over time. Previously, we have described a mouse model of POI that results from an oocyte-specific deletion of N- and O-glycans; Double Mutant (DM). DM females produce only one litter before undergoing POI due to ovarian dysfunction. In this study, we have characterised the gene expression profile of prepuberal (3 weeks), fertile (6 weeks) and infertile (9 weeks) DM ovaries. Up-regulation of cathepsin K (Ctsk, with unknown ovarian function) seems to trigger transcriptional changes in DM ovaries. Significant transcriptional changes then occur rapidly, associated with morphophysiological changes displayed by DM mice throughout the onset of POI. We identified genetic pathways such as extracellular matrix and immune response as candidates for the onset of POI in DM females. Remarkably, DM mice and POI women share a set of differentially expressed genes, including a functionally and co-expressed network of Mcm (minichromosome maintenance proteins) family members. The transcriptomic profile of the DM mouse model provides novel insight into the aetiology of POI. |
| first_indexed | 2024-03-07T07:48:28Z |
| format | Journal article |
| id | oxford-uuid:6d83a311-897a-4668-b186-15d998b5f50b |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:48:28Z |
| publishDate | 2022 |
| publisher | Bioscientifica |
| record_format | dspace |
| spelling | oxford-uuid:6d83a311-897a-4668-b186-15d998b5f50b2023-06-27T17:19:09ZRapid ovarian transcript changes during the onset of premature ovarian insufficiencyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6d83a311-897a-4668-b186-15d998b5f50bEnglishSymplectic ElementsBioscientifica2022Kaune, HMontiel, JFFenwick, MWilliams, SAPremature ovarian insufficiency (POI) affects 1-3% of women under 40 years of age. The identified causes are highly heterogeneous, and 70% of the cases are idiopathic. The ovarian manifestation varies from a variable population of follicles that fail to develop (follicular POI) to the absence of follicles (afollicular POI) with a transition from one to the other over time. Previously, we have described a mouse model of POI that results from an oocyte-specific deletion of N- and O-glycans; Double Mutant (DM). DM females produce only one litter before undergoing POI due to ovarian dysfunction. In this study, we have characterised the gene expression profile of prepuberal (3 weeks), fertile (6 weeks) and infertile (9 weeks) DM ovaries. Up-regulation of cathepsin K (Ctsk, with unknown ovarian function) seems to trigger transcriptional changes in DM ovaries. Significant transcriptional changes then occur rapidly, associated with morphophysiological changes displayed by DM mice throughout the onset of POI. We identified genetic pathways such as extracellular matrix and immune response as candidates for the onset of POI in DM females. Remarkably, DM mice and POI women share a set of differentially expressed genes, including a functionally and co-expressed network of Mcm (minichromosome maintenance proteins) family members. The transcriptomic profile of the DM mouse model provides novel insight into the aetiology of POI. |
| spellingShingle | Kaune, H Montiel, JF Fenwick, M Williams, SA Rapid ovarian transcript changes during the onset of premature ovarian insufficiency |
| title | Rapid ovarian transcript changes during the onset of premature ovarian insufficiency |
| title_full | Rapid ovarian transcript changes during the onset of premature ovarian insufficiency |
| title_fullStr | Rapid ovarian transcript changes during the onset of premature ovarian insufficiency |
| title_full_unstemmed | Rapid ovarian transcript changes during the onset of premature ovarian insufficiency |
| title_short | Rapid ovarian transcript changes during the onset of premature ovarian insufficiency |
| title_sort | rapid ovarian transcript changes during the onset of premature ovarian insufficiency |
| work_keys_str_mv | AT kauneh rapidovariantranscriptchangesduringtheonsetofprematureovarianinsufficiency AT montieljf rapidovariantranscriptchangesduringtheonsetofprematureovarianinsufficiency AT fenwickm rapidovariantranscriptchangesduringtheonsetofprematureovarianinsufficiency AT williamssa rapidovariantranscriptchangesduringtheonsetofprematureovarianinsufficiency |