Diagnostic utility of aP2/FABP4 expression in soft tissue tumours.
Adipocyte P2 (aP2), also known as fatty acid-binding protein 4 (FABP4), is a fatty acid-binding protein found in the cytoplasm of cells of adipocyte differentiation. In this study, we examined a large number of soft tissue tumours with a commercial polyclonal anti-aP2/FABP4 antibody and a newly deve...
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| Format: | Conference item |
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2013
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| author | Kashima, T Turley, H Dongre, A Pezzella, F Athanasou, N |
| author_facet | Kashima, T Turley, H Dongre, A Pezzella, F Athanasou, N |
| author_sort | Kashima, T |
| collection | OXFORD |
| description | Adipocyte P2 (aP2), also known as fatty acid-binding protein 4 (FABP4), is a fatty acid-binding protein found in the cytoplasm of cells of adipocyte differentiation. In this study, we examined a large number of soft tissue tumours with a commercial polyclonal anti-aP2/FABP4 antibody and a newly developed mouse monoclonal antibody raised against this protein to determine the diagnostic utility of aP2/FABP4 as a marker of tumours of adipose differentiation. A mouse monoclonal antibody, clone 175d, was raised against a mixture of synthetic peptides corresponding to the amino acid sequence of residues 10-28 and 121-132 of the human aP2/FABP4 protein. Antigen expression with polyclonal and monoclonal antibodies was immunohistochemically determined in paraffin sections of normal adipose tissue and a wide range of benign and malignant primary soft tissue tumours (n = 200). aP2/FABP4 was expressed around the cytoplasmic lipid vacuole in white and brown fat cells in benign lipomas and hibernomas. Immature fat cells and lipoblasts in spindle cell/pleomorphic lipoma, atypical lipomatous tumour/well-differentiated liposarcoma, myxoid/round cell liposarcoma and pleomorphic liposarcoma also reacted strongly for aP2/FABP4. No specific staining was seen in non-adipose benign and malignant mesenchymal and non-mesenchymal tumours. aP2/FABP4 is expressed by mature and immature fat cells and is a marker of tumours of adipose differentiation. Immunophenotypic aP2/FABP4 expression is useful in identifying lipoblasts, and immunohistochemistry with polyclonal/monoclonal anti-aP2/FABP4 antibodies should be useful in distinguishing liposarcoma from other malignancies, particularly round cell, myxoid and pleomorphic soft tissue sarcomas. |
| first_indexed | 2024-03-06T23:37:50Z |
| format | Conference item |
| id | oxford-uuid:6e45c12f-aca6-4f0b-8743-de16c33e1690 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T23:37:50Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:6e45c12f-aca6-4f0b-8743-de16c33e16902022-03-26T19:23:19ZDiagnostic utility of aP2/FABP4 expression in soft tissue tumours.Conference itemhttp://purl.org/coar/resource_type/c_5794uuid:6e45c12f-aca6-4f0b-8743-de16c33e1690Symplectic Elements at Oxford2013Kashima, TTurley, HDongre, APezzella, FAthanasou, NAdipocyte P2 (aP2), also known as fatty acid-binding protein 4 (FABP4), is a fatty acid-binding protein found in the cytoplasm of cells of adipocyte differentiation. In this study, we examined a large number of soft tissue tumours with a commercial polyclonal anti-aP2/FABP4 antibody and a newly developed mouse monoclonal antibody raised against this protein to determine the diagnostic utility of aP2/FABP4 as a marker of tumours of adipose differentiation. A mouse monoclonal antibody, clone 175d, was raised against a mixture of synthetic peptides corresponding to the amino acid sequence of residues 10-28 and 121-132 of the human aP2/FABP4 protein. Antigen expression with polyclonal and monoclonal antibodies was immunohistochemically determined in paraffin sections of normal adipose tissue and a wide range of benign and malignant primary soft tissue tumours (n = 200). aP2/FABP4 was expressed around the cytoplasmic lipid vacuole in white and brown fat cells in benign lipomas and hibernomas. Immature fat cells and lipoblasts in spindle cell/pleomorphic lipoma, atypical lipomatous tumour/well-differentiated liposarcoma, myxoid/round cell liposarcoma and pleomorphic liposarcoma also reacted strongly for aP2/FABP4. No specific staining was seen in non-adipose benign and malignant mesenchymal and non-mesenchymal tumours. aP2/FABP4 is expressed by mature and immature fat cells and is a marker of tumours of adipose differentiation. Immunophenotypic aP2/FABP4 expression is useful in identifying lipoblasts, and immunohistochemistry with polyclonal/monoclonal anti-aP2/FABP4 antibodies should be useful in distinguishing liposarcoma from other malignancies, particularly round cell, myxoid and pleomorphic soft tissue sarcomas. |
| spellingShingle | Kashima, T Turley, H Dongre, A Pezzella, F Athanasou, N Diagnostic utility of aP2/FABP4 expression in soft tissue tumours. |
| title | Diagnostic utility of aP2/FABP4 expression in soft tissue tumours. |
| title_full | Diagnostic utility of aP2/FABP4 expression in soft tissue tumours. |
| title_fullStr | Diagnostic utility of aP2/FABP4 expression in soft tissue tumours. |
| title_full_unstemmed | Diagnostic utility of aP2/FABP4 expression in soft tissue tumours. |
| title_short | Diagnostic utility of aP2/FABP4 expression in soft tissue tumours. |
| title_sort | diagnostic utility of ap2 fabp4 expression in soft tissue tumours |
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