Characterization of two Epstein-Barr virus epitopes restricted by HLA-B7.
We have identified two epitopes for Epstein-Barr virus specific cytotoxic T lymphocytes (CTL) restricted by the common allele HLA-B7. They are EBNA 3C 881-9 (QPRAPIRPI) and EBNA 3A 379-387 (RPPIFIRRL). The epitopes conform well to the recently described motif for HLA-B7-binding peptides (Huckzo et a...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
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1995
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| _version_ | 1797074670718025728 |
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| author | Hill, A Worth, A Elliott, T Rowland-Jones, S Brooks, J Rickinson, A McMichael, A |
| author_facet | Hill, A Worth, A Elliott, T Rowland-Jones, S Brooks, J Rickinson, A McMichael, A |
| author_sort | Hill, A |
| collection | OXFORD |
| description | We have identified two epitopes for Epstein-Barr virus specific cytotoxic T lymphocytes (CTL) restricted by the common allele HLA-B7. They are EBNA 3C 881-9 (QPRAPIRPI) and EBNA 3A 379-387 (RPPIFIRRL). The epitopes conform well to the recently described motif for HLA-B7-binding peptides (Huckzo et al., J. Immunol. 1993. 151:2572). Titration of the peptides in CTL assays and detergent lysate binding assays revealed that extending the peptides at either the N or C terminus did not reduce their affinity for HLA-B7. This behavior contrasted with HLA-B51, which binds peptides with a similar motif to B7, and has identical amino acid residues at sites expected to form the "F" pocket of the peptide-binding groove. HLA-B51 also bound the peptide EBNA 3C 881-9, but was unable to bind peptides extended at the C terminus. |
| first_indexed | 2024-03-06T23:39:36Z |
| format | Journal article |
| id | oxford-uuid:6ed6eb3a-4b6c-42b7-8d49-644be4e0fe5b |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:39:36Z |
| publishDate | 1995 |
| record_format | dspace |
| spelling | oxford-uuid:6ed6eb3a-4b6c-42b7-8d49-644be4e0fe5b2022-03-26T19:27:01ZCharacterization of two Epstein-Barr virus epitopes restricted by HLA-B7.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6ed6eb3a-4b6c-42b7-8d49-644be4e0fe5bEnglishSymplectic Elements at Oxford1995Hill, AWorth, AElliott, TRowland-Jones, SBrooks, JRickinson, AMcMichael, AWe have identified two epitopes for Epstein-Barr virus specific cytotoxic T lymphocytes (CTL) restricted by the common allele HLA-B7. They are EBNA 3C 881-9 (QPRAPIRPI) and EBNA 3A 379-387 (RPPIFIRRL). The epitopes conform well to the recently described motif for HLA-B7-binding peptides (Huckzo et al., J. Immunol. 1993. 151:2572). Titration of the peptides in CTL assays and detergent lysate binding assays revealed that extending the peptides at either the N or C terminus did not reduce their affinity for HLA-B7. This behavior contrasted with HLA-B51, which binds peptides with a similar motif to B7, and has identical amino acid residues at sites expected to form the "F" pocket of the peptide-binding groove. HLA-B51 also bound the peptide EBNA 3C 881-9, but was unable to bind peptides extended at the C terminus. |
| spellingShingle | Hill, A Worth, A Elliott, T Rowland-Jones, S Brooks, J Rickinson, A McMichael, A Characterization of two Epstein-Barr virus epitopes restricted by HLA-B7. |
| title | Characterization of two Epstein-Barr virus epitopes restricted by HLA-B7. |
| title_full | Characterization of two Epstein-Barr virus epitopes restricted by HLA-B7. |
| title_fullStr | Characterization of two Epstein-Barr virus epitopes restricted by HLA-B7. |
| title_full_unstemmed | Characterization of two Epstein-Barr virus epitopes restricted by HLA-B7. |
| title_short | Characterization of two Epstein-Barr virus epitopes restricted by HLA-B7. |
| title_sort | characterization of two epstein barr virus epitopes restricted by hla b7 |
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