Antigen-specific and non-specific helper activities derived from supernatants of human influenza virus-specific T-cell lines.
A T-cell line (H3) was established by culturing human peripheral blood mononuclear cells with influenza virus A/X31 and maintained in long term culture with Interleukin-2 (TCGF). Supernatants were prepared by culturing these cells overnight in the absence of Interleukin-2 but with A/X31 and irradiat...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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1983
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| _version_ | 1826278156293636096 |
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| author | Zanders, E Fischer, A Smith, S Beverley, P Feldmann, M |
| author_facet | Zanders, E Fischer, A Smith, S Beverley, P Feldmann, M |
| author_sort | Zanders, E |
| collection | OXFORD |
| description | A T-cell line (H3) was established by culturing human peripheral blood mononuclear cells with influenza virus A/X31 and maintained in long term culture with Interleukin-2 (TCGF). Supernatants were prepared by culturing these cells overnight in the absence of Interleukin-2 but with A/X31 and irradiated autologous E rosette negative cells as a source of antigen presenting cells, and harvesting by centrifugation. The supernatants were shown to replace T cells in helping E- (B) cells to produce antibody specific to A/X31 which was measured by enzyme immunoassay (EIA). Although maximal help was obtained with autologous or semi allogeneic B cells (in the latter case bearing HLA-DR 3 loci) there was still significant antibody production with allogeneic combinations. The supernatants were subsequently fractionated into specific and non-specific helper activities by gel filtration, giving an approximate mol. wt of 50-70,000 and 10-30,000 for each respectively. The specific HF was shown to be genetically restricted in its action upon B cells and also to generate antibody to A/X31 only. The lower molecular weight material acted on any responding B cell regardless of HLA-DR type and produced antibody non-specifically in culture with E- cells even in the absence of antigen. The apparent lack of restriction was therefore due to the masking effect of non-specific and non-restricted HF(s) on the genetically restricted specific HF produced by this line. |
| first_indexed | 2024-03-06T23:39:44Z |
| format | Journal article |
| id | oxford-uuid:6ee1d52b-79f4-46cb-8442-943eb15bef6b |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:39:44Z |
| publishDate | 1983 |
| record_format | dspace |
| spelling | oxford-uuid:6ee1d52b-79f4-46cb-8442-943eb15bef6b2022-03-26T19:27:15ZAntigen-specific and non-specific helper activities derived from supernatants of human influenza virus-specific T-cell lines.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6ee1d52b-79f4-46cb-8442-943eb15bef6bEnglishSymplectic Elements at Oxford1983Zanders, EFischer, ASmith, SBeverley, PFeldmann, MA T-cell line (H3) was established by culturing human peripheral blood mononuclear cells with influenza virus A/X31 and maintained in long term culture with Interleukin-2 (TCGF). Supernatants were prepared by culturing these cells overnight in the absence of Interleukin-2 but with A/X31 and irradiated autologous E rosette negative cells as a source of antigen presenting cells, and harvesting by centrifugation. The supernatants were shown to replace T cells in helping E- (B) cells to produce antibody specific to A/X31 which was measured by enzyme immunoassay (EIA). Although maximal help was obtained with autologous or semi allogeneic B cells (in the latter case bearing HLA-DR 3 loci) there was still significant antibody production with allogeneic combinations. The supernatants were subsequently fractionated into specific and non-specific helper activities by gel filtration, giving an approximate mol. wt of 50-70,000 and 10-30,000 for each respectively. The specific HF was shown to be genetically restricted in its action upon B cells and also to generate antibody to A/X31 only. The lower molecular weight material acted on any responding B cell regardless of HLA-DR type and produced antibody non-specifically in culture with E- cells even in the absence of antigen. The apparent lack of restriction was therefore due to the masking effect of non-specific and non-restricted HF(s) on the genetically restricted specific HF produced by this line. |
| spellingShingle | Zanders, E Fischer, A Smith, S Beverley, P Feldmann, M Antigen-specific and non-specific helper activities derived from supernatants of human influenza virus-specific T-cell lines. |
| title | Antigen-specific and non-specific helper activities derived from supernatants of human influenza virus-specific T-cell lines. |
| title_full | Antigen-specific and non-specific helper activities derived from supernatants of human influenza virus-specific T-cell lines. |
| title_fullStr | Antigen-specific and non-specific helper activities derived from supernatants of human influenza virus-specific T-cell lines. |
| title_full_unstemmed | Antigen-specific and non-specific helper activities derived from supernatants of human influenza virus-specific T-cell lines. |
| title_short | Antigen-specific and non-specific helper activities derived from supernatants of human influenza virus-specific T-cell lines. |
| title_sort | antigen specific and non specific helper activities derived from supernatants of human influenza virus specific t cell lines |
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