Characterization of the repertoire diversity of the Plasmodium falciparum stevor multigene family in laboratory and field isolates.
BACKGROUND: The evasion of host immune response by the human malaria parasite Plasmodium falciparum has been linked to expression of a range of variable antigens on the infected erythrocyte surface. Several genes are potentially involved in this process with the var, rif and stevor multigene familie...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
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BioMed Central
2009
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| _version_ | 1797074680053497856 |
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| author | Blythe, J Niang, M Marsh, K Holder, A Langhorne, J Preiser, P |
| author_facet | Blythe, J Niang, M Marsh, K Holder, A Langhorne, J Preiser, P |
| author_sort | Blythe, J |
| collection | OXFORD |
| description | BACKGROUND: The evasion of host immune response by the human malaria parasite Plasmodium falciparum has been linked to expression of a range of variable antigens on the infected erythrocyte surface. Several genes are potentially involved in this process with the var, rif and stevor multigene families being the most likely candidates and coding for rapidly evolving proteins. The high sequence diversity of proteins encoded by these gene families may have evolved as an immune evasion strategy that enables the parasite to establish long lasting chronic infections. Previous findings have shown that the hypervariable region (HVR) of STEVOR has significant sequence diversity both within as well as across different P. falciparum lines. However, these studies did not address whether or not there are ancestral stevor that can be found in different parasites. METHODS: DNA and RNA sequences analysis as well as phylogenetic approaches were used to analyse the stevor sequence repertoire and diversity in laboratory lines and Kilifi (Kenya) fresh isolates. RESULTS: Conserved stevor genes were identified in different P. falciparum isolates from different global locations. Consistent with previous studies, the HVR of the stevor gene family was found to be highly divergent both within and between isolates. Importantly phylogenetic analysis shows some clustering of stevor sequences both within a single parasite clone as well as across different parasite isolates. CONCLUSION: This indicates that the ancestral P. falciparum parasite genome already contained multiple stevor genes that have subsequently diversified further within the different P. falciparum populations. It also confirms that STEVOR is under strong selection pressure. |
| first_indexed | 2024-03-06T23:39:45Z |
| format | Journal article |
| id | oxford-uuid:6ee3dedc-5adc-4215-8791-411e5fdd1508 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:39:45Z |
| publishDate | 2009 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | oxford-uuid:6ee3dedc-5adc-4215-8791-411e5fdd15082022-03-26T19:27:17ZCharacterization of the repertoire diversity of the Plasmodium falciparum stevor multigene family in laboratory and field isolates.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6ee3dedc-5adc-4215-8791-411e5fdd1508EnglishSymplectic Elements at OxfordBioMed Central2009Blythe, JNiang, MMarsh, KHolder, ALanghorne, JPreiser, PBACKGROUND: The evasion of host immune response by the human malaria parasite Plasmodium falciparum has been linked to expression of a range of variable antigens on the infected erythrocyte surface. Several genes are potentially involved in this process with the var, rif and stevor multigene families being the most likely candidates and coding for rapidly evolving proteins. The high sequence diversity of proteins encoded by these gene families may have evolved as an immune evasion strategy that enables the parasite to establish long lasting chronic infections. Previous findings have shown that the hypervariable region (HVR) of STEVOR has significant sequence diversity both within as well as across different P. falciparum lines. However, these studies did not address whether or not there are ancestral stevor that can be found in different parasites. METHODS: DNA and RNA sequences analysis as well as phylogenetic approaches were used to analyse the stevor sequence repertoire and diversity in laboratory lines and Kilifi (Kenya) fresh isolates. RESULTS: Conserved stevor genes were identified in different P. falciparum isolates from different global locations. Consistent with previous studies, the HVR of the stevor gene family was found to be highly divergent both within and between isolates. Importantly phylogenetic analysis shows some clustering of stevor sequences both within a single parasite clone as well as across different parasite isolates. CONCLUSION: This indicates that the ancestral P. falciparum parasite genome already contained multiple stevor genes that have subsequently diversified further within the different P. falciparum populations. It also confirms that STEVOR is under strong selection pressure. |
| spellingShingle | Blythe, J Niang, M Marsh, K Holder, A Langhorne, J Preiser, P Characterization of the repertoire diversity of the Plasmodium falciparum stevor multigene family in laboratory and field isolates. |
| title | Characterization of the repertoire diversity of the Plasmodium falciparum stevor multigene family in laboratory and field isolates. |
| title_full | Characterization of the repertoire diversity of the Plasmodium falciparum stevor multigene family in laboratory and field isolates. |
| title_fullStr | Characterization of the repertoire diversity of the Plasmodium falciparum stevor multigene family in laboratory and field isolates. |
| title_full_unstemmed | Characterization of the repertoire diversity of the Plasmodium falciparum stevor multigene family in laboratory and field isolates. |
| title_short | Characterization of the repertoire diversity of the Plasmodium falciparum stevor multigene family in laboratory and field isolates. |
| title_sort | characterization of the repertoire diversity of the plasmodium falciparum stevor multigene family in laboratory and field isolates |
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