Interplay between recombination, cell division and chromosome structure during chromosome dimer resolution in Escherichia coli.
Chromosome dimers form in bacteria by recombination between circular chromosomes. Resolution of dimers is a highly integrated process involving recombination between dif sites catalysed by the XerCD recombinase, cell division and the integrity of the division septum-associated FtsK protein and the p...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2001
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| _version_ | 1826278370250326016 |
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| author | Pérals, K Capiaux, H Vincourt, J Louarn, J Sherratt, D Cornet, F |
| author_facet | Pérals, K Capiaux, H Vincourt, J Louarn, J Sherratt, D Cornet, F |
| author_sort | Pérals, K |
| collection | OXFORD |
| description | Chromosome dimers form in bacteria by recombination between circular chromosomes. Resolution of dimers is a highly integrated process involving recombination between dif sites catalysed by the XerCD recombinase, cell division and the integrity of the division septum-associated FtsK protein and the presence of dif inside a restricted region of the chromosome terminus, the dif activity zone (DAZ). We analyse here how these phenomena collaborate. We show that (i) both inter- and intrachromosomal recombination between dif sites are activated by their presence inside the DAZ; (ii) the DAZ-specific activation only occurs in conditions supporting the formation of chromosome dimers; (iii) overexpression of FtsK leads to a general increase in dif recombination irrespective of dif location; (iv) overexpression of FtsK does not improve the ability of dif sites inserted outside the DAZ to resolve chromosome dimers. Our results suggest that the formation of an active XerCD-FtsK-dif complex is restricted to when a dimer is present, the features of chromosome organization that determine the DAZ playing a central role in this control. |
| first_indexed | 2024-03-06T23:42:55Z |
| format | Journal article |
| id | oxford-uuid:6feb163d-dac6-4e88-bf01-4ea42779cf49 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:42:55Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:6feb163d-dac6-4e88-bf01-4ea42779cf492022-03-26T19:33:51ZInterplay between recombination, cell division and chromosome structure during chromosome dimer resolution in Escherichia coli.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6feb163d-dac6-4e88-bf01-4ea42779cf49EnglishSymplectic Elements at Oxford2001Pérals, KCapiaux, HVincourt, JLouarn, JSherratt, DCornet, FChromosome dimers form in bacteria by recombination between circular chromosomes. Resolution of dimers is a highly integrated process involving recombination between dif sites catalysed by the XerCD recombinase, cell division and the integrity of the division septum-associated FtsK protein and the presence of dif inside a restricted region of the chromosome terminus, the dif activity zone (DAZ). We analyse here how these phenomena collaborate. We show that (i) both inter- and intrachromosomal recombination between dif sites are activated by their presence inside the DAZ; (ii) the DAZ-specific activation only occurs in conditions supporting the formation of chromosome dimers; (iii) overexpression of FtsK leads to a general increase in dif recombination irrespective of dif location; (iv) overexpression of FtsK does not improve the ability of dif sites inserted outside the DAZ to resolve chromosome dimers. Our results suggest that the formation of an active XerCD-FtsK-dif complex is restricted to when a dimer is present, the features of chromosome organization that determine the DAZ playing a central role in this control. |
| spellingShingle | Pérals, K Capiaux, H Vincourt, J Louarn, J Sherratt, D Cornet, F Interplay between recombination, cell division and chromosome structure during chromosome dimer resolution in Escherichia coli. |
| title | Interplay between recombination, cell division and chromosome structure during chromosome dimer resolution in Escherichia coli. |
| title_full | Interplay between recombination, cell division and chromosome structure during chromosome dimer resolution in Escherichia coli. |
| title_fullStr | Interplay between recombination, cell division and chromosome structure during chromosome dimer resolution in Escherichia coli. |
| title_full_unstemmed | Interplay between recombination, cell division and chromosome structure during chromosome dimer resolution in Escherichia coli. |
| title_short | Interplay between recombination, cell division and chromosome structure during chromosome dimer resolution in Escherichia coli. |
| title_sort | interplay between recombination cell division and chromosome structure during chromosome dimer resolution in escherichia coli |
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