Modern solutions for ancient pathogens: direct pathogen sequencing for diagnosis of lepromatous leprosy and cerebral coenurosis 

Microbes unculturable in vitro remain diagnostically challenging, dependent historically on clinical findings, histology, or targeted molecular detection. We applied whole-genome sequencing directly from tissue to diagnose infections with mycobacteria (leprosy) and parasites (coenurosis). Direct pat...

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Bibliographic Details
Main Authors: Young, B, Bush, S, Lipworth, S, George, S, Dingle, K, Sanderson, N, brankin, A, Walker, T, Sharma, S, Leong, J, Plaha, P, Hofer, M, Chiodini, P, Gottstein, B, Furrer, L, Crook, D, Brent, A
Format: Journal article
Language:English
Published: Oxford University Press 2022
Description
Summary:Microbes unculturable in vitro remain diagnostically challenging, dependent historically on clinical findings, histology, or targeted molecular detection. We applied whole-genome sequencing directly from tissue to diagnose infections with mycobacteria (leprosy) and parasites (coenurosis). Direct pathogen DNA sequencing provides flexible solutions to diagnosis of difficult pathogens in diverse contexts.