The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility
Regulatory switches are wide spread in many biological systems. Uniquely among them, the switch of the bacterial flagellar motor is not an on/off switch but rather controls the motor's direction of rotation in response to binding of the signaling protein CheY. Despite its extensive study, the m...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
EMBO Press
2021
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| _version_ | 1797074922097344512 |
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| author | Afanzar, O Di Paolo, D Eisenstein, M Levi, K Plochowietz, A Kapanidis, AN Berry, RM Eisenbach, M |
| author_facet | Afanzar, O Di Paolo, D Eisenstein, M Levi, K Plochowietz, A Kapanidis, AN Berry, RM Eisenbach, M |
| author_sort | Afanzar, O |
| collection | OXFORD |
| description | Regulatory switches are wide spread in many biological systems. Uniquely among them, the switch of the bacterial flagellar motor is not an on/off switch but rather controls the motor's direction of rotation in response to binding of the signaling protein CheY. Despite its extensive study, the molecular mechanism underlying this switch has remained largely unclear. Here, we resolved the functions of each of the three CheY-binding sites at the switch in E. coli, as well as their different dependencies on phosphorylation and acetylation of CheY. Based on this, we propose that CheY motor switching activity is potentiated upon binding to the first site. Binding of potentiated CheY to the second site produces unstable switching and at the same time enables CheY binding to the third site, an event that stabilizes the switched state. Thereby, this mechanism exemplifies a unique combination of tight motor regulation with inherent switching flexibility. |
| first_indexed | 2024-03-06T23:43:15Z |
| format | Journal article |
| id | oxford-uuid:700a1292-1bfe-4b30-b4f6-d221429dc56d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:43:15Z |
| publishDate | 2021 |
| publisher | EMBO Press |
| record_format | dspace |
| spelling | oxford-uuid:700a1292-1bfe-4b30-b4f6-d221429dc56d2022-03-26T19:34:35ZThe switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibilityJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:700a1292-1bfe-4b30-b4f6-d221429dc56dEnglishSymplectic ElementsEMBO Press2021Afanzar, ODi Paolo, DEisenstein, MLevi, KPlochowietz, AKapanidis, ANBerry, RMEisenbach, MRegulatory switches are wide spread in many biological systems. Uniquely among them, the switch of the bacterial flagellar motor is not an on/off switch but rather controls the motor's direction of rotation in response to binding of the signaling protein CheY. Despite its extensive study, the molecular mechanism underlying this switch has remained largely unclear. Here, we resolved the functions of each of the three CheY-binding sites at the switch in E. coli, as well as their different dependencies on phosphorylation and acetylation of CheY. Based on this, we propose that CheY motor switching activity is potentiated upon binding to the first site. Binding of potentiated CheY to the second site produces unstable switching and at the same time enables CheY binding to the third site, an event that stabilizes the switched state. Thereby, this mechanism exemplifies a unique combination of tight motor regulation with inherent switching flexibility. |
| spellingShingle | Afanzar, O Di Paolo, D Eisenstein, M Levi, K Plochowietz, A Kapanidis, AN Berry, RM Eisenbach, M The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility |
| title | The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility |
| title_full | The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility |
| title_fullStr | The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility |
| title_full_unstemmed | The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility |
| title_short | The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility |
| title_sort | switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility |
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