The mouse Runx1 +23 hematopoietic stem cell enhancer confers hematopoietic specificity to both Runx1 promoters.
The transcription factor Runx1 plays a pivotal role in hematopoietic stem cell (HSC) emergence, and studies into its transcriptional regulation should give insight into the critical steps of HSC specification. Recently, we identified the Runx1 +23 enhancer that targets reporter gene expression to th...
| Autors principals: | , , , , , , , |
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| Format: | Journal article |
| Idioma: | English |
| Publicat: |
2009
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| _version_ | 1826278487326982144 |
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| author | Bee, T Ashley, E Bickley, SR Jarratt, A Li, P Sloane-Stanley, J Göttgens, B de Bruijn, M |
| author_facet | Bee, T Ashley, E Bickley, SR Jarratt, A Li, P Sloane-Stanley, J Göttgens, B de Bruijn, M |
| author_sort | Bee, T |
| collection | OXFORD |
| description | The transcription factor Runx1 plays a pivotal role in hematopoietic stem cell (HSC) emergence, and studies into its transcriptional regulation should give insight into the critical steps of HSC specification. Recently, we identified the Runx1 +23 enhancer that targets reporter gene expression to the first emerging HSCs of the mouse embryo when linked to the heterologous hsp68 promoter. Endogenous Runx1 is transcribed from 2 alternative promoters, P1 and P2. Here, we examined the in vivo cis-regulatory potential of these alternative promoters and asked whether they act with and contribute to the spatiotemporal specific expression of the Runx1 +23 enhancer. Our results firmly establish that, in contrast to zebrafish runx1, mouse Runx1 promoter sequences do not confer any hematopoietic specificity in transgenic embryos. Yet, both mouse promoters act with the +23 enhancer to drive reporter gene expression to sites of HSC emergence and colonization, in a +23-specific pattern. |
| first_indexed | 2024-03-06T23:44:39Z |
| format | Journal article |
| id | oxford-uuid:707b2f8c-ddf1-4afc-8fc9-3a5450ccf6a5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:44:39Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:707b2f8c-ddf1-4afc-8fc9-3a5450ccf6a52022-03-26T19:37:28ZThe mouse Runx1 +23 hematopoietic stem cell enhancer confers hematopoietic specificity to both Runx1 promoters.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:707b2f8c-ddf1-4afc-8fc9-3a5450ccf6a5EnglishSymplectic Elements at Oxford2009Bee, TAshley, EBickley, SRJarratt, ALi, PSloane-Stanley, JGöttgens, Bde Bruijn, MThe transcription factor Runx1 plays a pivotal role in hematopoietic stem cell (HSC) emergence, and studies into its transcriptional regulation should give insight into the critical steps of HSC specification. Recently, we identified the Runx1 +23 enhancer that targets reporter gene expression to the first emerging HSCs of the mouse embryo when linked to the heterologous hsp68 promoter. Endogenous Runx1 is transcribed from 2 alternative promoters, P1 and P2. Here, we examined the in vivo cis-regulatory potential of these alternative promoters and asked whether they act with and contribute to the spatiotemporal specific expression of the Runx1 +23 enhancer. Our results firmly establish that, in contrast to zebrafish runx1, mouse Runx1 promoter sequences do not confer any hematopoietic specificity in transgenic embryos. Yet, both mouse promoters act with the +23 enhancer to drive reporter gene expression to sites of HSC emergence and colonization, in a +23-specific pattern. |
| spellingShingle | Bee, T Ashley, E Bickley, SR Jarratt, A Li, P Sloane-Stanley, J Göttgens, B de Bruijn, M The mouse Runx1 +23 hematopoietic stem cell enhancer confers hematopoietic specificity to both Runx1 promoters. |
| title | The mouse Runx1 +23 hematopoietic stem cell enhancer confers hematopoietic specificity to both Runx1 promoters. |
| title_full | The mouse Runx1 +23 hematopoietic stem cell enhancer confers hematopoietic specificity to both Runx1 promoters. |
| title_fullStr | The mouse Runx1 +23 hematopoietic stem cell enhancer confers hematopoietic specificity to both Runx1 promoters. |
| title_full_unstemmed | The mouse Runx1 +23 hematopoietic stem cell enhancer confers hematopoietic specificity to both Runx1 promoters. |
| title_short | The mouse Runx1 +23 hematopoietic stem cell enhancer confers hematopoietic specificity to both Runx1 promoters. |
| title_sort | mouse runx1 23 hematopoietic stem cell enhancer confers hematopoietic specificity to both runx1 promoters |
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