Proteomic analysis of HepaRG cells: a novel cell line that supports hepatitis B virus infection.
The first proteomic characterization of the HepaRG cell line, the only cell line that is susceptible to hepatitis B virus (HBV) infection and supports a complete virus life cycle, is reported. Differential analysis of naive and HBV-infected HepaRG cells by two-dimensional gel electrophoresis reveale...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
American Chemical Society
2009
|
| _version_ | 1797075102480728064 |
|---|---|
| author | Narayan, R Gangadharan, B Hantz, O Antrobus, R García, Á Dwek, R Zitzmann, N |
| author_facet | Narayan, R Gangadharan, B Hantz, O Antrobus, R García, Á Dwek, R Zitzmann, N |
| author_sort | Narayan, R |
| collection | OXFORD |
| description | The first proteomic characterization of the HepaRG cell line, the only cell line that is susceptible to hepatitis B virus (HBV) infection and supports a complete virus life cycle, is reported. Differential analysis of naive and HBV-infected HepaRG cells by two-dimensional gel electrophoresis revealed 19 differentially regulated features, 7 increasing and 12 decreasing with HBV infection. The proteins identified in these features were involved in various cellular pathways including apoptosis, DNA/RNA processing, and hepatocellular impairment. Similar expression changes in a number of the identified proteins have already been reported for other virus systems. Identification of these expression changes is a validation of the proteomics approach and contributes to an understanding of host cellular response to HBV infection. |
| first_indexed | 2024-03-06T23:45:33Z |
| format | Journal article |
| id | oxford-uuid:70c5bd49-8413-4201-a0b7-65051cb570b4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:45:33Z |
| publishDate | 2009 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:70c5bd49-8413-4201-a0b7-65051cb570b42022-03-26T19:39:24ZProteomic analysis of HepaRG cells: a novel cell line that supports hepatitis B virus infection.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:70c5bd49-8413-4201-a0b7-65051cb570b4EnglishSymplectic Elements at OxfordAmerican Chemical Society2009Narayan, RGangadharan, BHantz, OAntrobus, RGarcía, ÁDwek, RZitzmann, NThe first proteomic characterization of the HepaRG cell line, the only cell line that is susceptible to hepatitis B virus (HBV) infection and supports a complete virus life cycle, is reported. Differential analysis of naive and HBV-infected HepaRG cells by two-dimensional gel electrophoresis revealed 19 differentially regulated features, 7 increasing and 12 decreasing with HBV infection. The proteins identified in these features were involved in various cellular pathways including apoptosis, DNA/RNA processing, and hepatocellular impairment. Similar expression changes in a number of the identified proteins have already been reported for other virus systems. Identification of these expression changes is a validation of the proteomics approach and contributes to an understanding of host cellular response to HBV infection. |
| spellingShingle | Narayan, R Gangadharan, B Hantz, O Antrobus, R García, Á Dwek, R Zitzmann, N Proteomic analysis of HepaRG cells: a novel cell line that supports hepatitis B virus infection. |
| title | Proteomic analysis of HepaRG cells: a novel cell line that supports hepatitis B virus infection. |
| title_full | Proteomic analysis of HepaRG cells: a novel cell line that supports hepatitis B virus infection. |
| title_fullStr | Proteomic analysis of HepaRG cells: a novel cell line that supports hepatitis B virus infection. |
| title_full_unstemmed | Proteomic analysis of HepaRG cells: a novel cell line that supports hepatitis B virus infection. |
| title_short | Proteomic analysis of HepaRG cells: a novel cell line that supports hepatitis B virus infection. |
| title_sort | proteomic analysis of heparg cells a novel cell line that supports hepatitis b virus infection |
| work_keys_str_mv | AT narayanr proteomicanalysisofhepargcellsanovelcelllinethatsupportshepatitisbvirusinfection AT gangadharanb proteomicanalysisofhepargcellsanovelcelllinethatsupportshepatitisbvirusinfection AT hantzo proteomicanalysisofhepargcellsanovelcelllinethatsupportshepatitisbvirusinfection AT antrobusr proteomicanalysisofhepargcellsanovelcelllinethatsupportshepatitisbvirusinfection AT garciaa proteomicanalysisofhepargcellsanovelcelllinethatsupportshepatitisbvirusinfection AT dwekr proteomicanalysisofhepargcellsanovelcelllinethatsupportshepatitisbvirusinfection AT zitzmannn proteomicanalysisofhepargcellsanovelcelllinethatsupportshepatitisbvirusinfection |