A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein
Immunization with attenuated Plasmodium falciparum sporozoites (PfSPZs) has been shown to be protective against malaria, but the features of the antibody response induced by this treatment remain unclear. To investigate this response in detail, we isolated IgM and IgG monoclonal antibodies from Tanz...
| Hlavní autoři: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Médium: | Journal article |
| Jazyk: | English |
| Vydáno: |
Springer Nature
2018
|
| _version_ | 1826278617310560256 |
|---|---|
| author | Tan, J Sack, B Oyen, D Zenklusen, I Piccoli, L Barbieri, S Foglierini, M Fregni, C Marcandalli, J Jongo, S Abdulla, S Perez, L Corradin, G Varani, L Sallusto, F Sim, B Hoffman, S Kappe, S Daubenberger, C Wilson, I Lanzavecchia, A |
| author_facet | Tan, J Sack, B Oyen, D Zenklusen, I Piccoli, L Barbieri, S Foglierini, M Fregni, C Marcandalli, J Jongo, S Abdulla, S Perez, L Corradin, G Varani, L Sallusto, F Sim, B Hoffman, S Kappe, S Daubenberger, C Wilson, I Lanzavecchia, A |
| author_sort | Tan, J |
| collection | OXFORD |
| description | Immunization with attenuated Plasmodium falciparum sporozoites (PfSPZs) has been shown to be protective against malaria, but the features of the antibody response induced by this treatment remain unclear. To investigate this response in detail, we isolated IgM and IgG monoclonal antibodies from Tanzanian volunteers who were immunized with repeated injection of Sanaria PfSPZ Vaccine and who were found to be protected from controlled human malaria infection with infectious homologous PfSPZs. All isolated IgG monoclonal antibodies bound to P. falciparum circumsporozoite protein (PfCSP) and recognized distinct epitopes in its N terminus, NANP-repeat region, and C terminus. Strikingly, the most effective antibodies, as determined in a humanized mouse model, bound not only to the repeat region, but also to a minimal peptide at the PfCSP N-terminal junction that is not in the RTS,S vaccine. These dual-specific antibodies were isolated from different donors and were encoded by VH3-30 or VH3-33 alleles that encode tryptophan or arginine at position 52. Using structural and mutational data, we describe the elements required for germline recognition and affinity maturation. Our study provides potent neutralizing antibodies and relevant information for lineage-targeted vaccine design and immunization strategies. |
| first_indexed | 2024-03-06T23:46:37Z |
| format | Journal article |
| id | oxford-uuid:71230ed8-5d15-4ef8-b00c-3c344c83c3a1 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:46:37Z |
| publishDate | 2018 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | oxford-uuid:71230ed8-5d15-4ef8-b00c-3c344c83c3a12022-03-26T19:41:39ZA public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite proteinJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:71230ed8-5d15-4ef8-b00c-3c344c83c3a1EnglishSymplectic Elements at OxfordSpringer Nature2018Tan, JSack, BOyen, DZenklusen, IPiccoli, LBarbieri, SFoglierini, MFregni, CMarcandalli, JJongo, SAbdulla, SPerez, LCorradin, GVarani, LSallusto, FSim, BHoffman, SKappe, SDaubenberger, CWilson, ILanzavecchia, AImmunization with attenuated Plasmodium falciparum sporozoites (PfSPZs) has been shown to be protective against malaria, but the features of the antibody response induced by this treatment remain unclear. To investigate this response in detail, we isolated IgM and IgG monoclonal antibodies from Tanzanian volunteers who were immunized with repeated injection of Sanaria PfSPZ Vaccine and who were found to be protected from controlled human malaria infection with infectious homologous PfSPZs. All isolated IgG monoclonal antibodies bound to P. falciparum circumsporozoite protein (PfCSP) and recognized distinct epitopes in its N terminus, NANP-repeat region, and C terminus. Strikingly, the most effective antibodies, as determined in a humanized mouse model, bound not only to the repeat region, but also to a minimal peptide at the PfCSP N-terminal junction that is not in the RTS,S vaccine. These dual-specific antibodies were isolated from different donors and were encoded by VH3-30 or VH3-33 alleles that encode tryptophan or arginine at position 52. Using structural and mutational data, we describe the elements required for germline recognition and affinity maturation. Our study provides potent neutralizing antibodies and relevant information for lineage-targeted vaccine design and immunization strategies. |
| spellingShingle | Tan, J Sack, B Oyen, D Zenklusen, I Piccoli, L Barbieri, S Foglierini, M Fregni, C Marcandalli, J Jongo, S Abdulla, S Perez, L Corradin, G Varani, L Sallusto, F Sim, B Hoffman, S Kappe, S Daubenberger, C Wilson, I Lanzavecchia, A A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein |
| title | A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein |
| title_full | A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein |
| title_fullStr | A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein |
| title_full_unstemmed | A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein |
| title_short | A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein |
| title_sort | public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein |
| work_keys_str_mv | AT tanj apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT sackb apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT oyend apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT zenkluseni apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT piccolil apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT barbieris apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT foglierinim apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT fregnic apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT marcandallij apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT jongos apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT abdullas apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT perezl apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT corrading apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT varanil apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT sallustof apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT simb apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT hoffmans apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT kappes apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT daubenbergerc apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT wilsoni apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT lanzavecchiaa apublicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT tanj publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT sackb publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT oyend publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT zenkluseni publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT piccolil publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT barbieris publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT foglierinim publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT fregnic publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT marcandallij publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT jongos publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT abdullas publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT perezl publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT corrading publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT varanil publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT sallustof publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT simb publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT hoffmans publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT kappes publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT daubenbergerc publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT wilsoni publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein AT lanzavecchiaa publicantibodylineagethatpotentlyinhibitsmalariainfectionthroughdualbindingtothecircumsporozoiteprotein |