Complete mapping of a novel HLA A*6801-restricted HIV-1 Tat epitope directly with a rapid modified enzyme-linked immunospot assay.
We identified a novel HLA A*6801-restricted HIV-1 Tat-derived cytotoxic T lymphocyte (CTL) epitope using an adapted enzyme-linked immunospot assay that allows the rapid ex vivo identification of CTL epitopes together with their associated HLA Class I restriction elements. The optimal 11 amino acid r...
| Principais autores: | , , , , , , , , , |
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| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
2002
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| _version_ | 1826278696546205696 |
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| author | Oxenius, A Jakobsen, B Easterbrook, P Boulter, J Tun, T Waters, A Agudelo, J Barnardo, M Phillips, R Price, D |
| author_facet | Oxenius, A Jakobsen, B Easterbrook, P Boulter, J Tun, T Waters, A Agudelo, J Barnardo, M Phillips, R Price, D |
| author_sort | Oxenius, A |
| collection | OXFORD |
| description | We identified a novel HLA A*6801-restricted HIV-1 Tat-derived cytotoxic T lymphocyte (CTL) epitope using an adapted enzyme-linked immunospot assay that allows the rapid ex vivo identification of CTL epitopes together with their associated HLA Class I restriction elements. The optimal 11 amino acid residue Tat epitope efficiently stabilized the refolding of monomeric peptide-HLA A6801 complexes in vitro and fluorochrome-labelled, tetrameric peptide-HLA A6801 complexes stained CD8 T cells specific for this epitope directly ex vivo. |
| first_indexed | 2024-03-06T23:47:48Z |
| format | Journal article |
| id | oxford-uuid:718b71d0-9e93-4b09-a0a7-4a17525d8e09 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:47:48Z |
| publishDate | 2002 |
| record_format | dspace |
| spelling | oxford-uuid:718b71d0-9e93-4b09-a0a7-4a17525d8e092022-03-26T19:44:22ZComplete mapping of a novel HLA A*6801-restricted HIV-1 Tat epitope directly with a rapid modified enzyme-linked immunospot assay.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:718b71d0-9e93-4b09-a0a7-4a17525d8e09EnglishSymplectic Elements at Oxford2002Oxenius, AJakobsen, BEasterbrook, PBoulter, JTun, TWaters, AAgudelo, JBarnardo, MPhillips, RPrice, DWe identified a novel HLA A*6801-restricted HIV-1 Tat-derived cytotoxic T lymphocyte (CTL) epitope using an adapted enzyme-linked immunospot assay that allows the rapid ex vivo identification of CTL epitopes together with their associated HLA Class I restriction elements. The optimal 11 amino acid residue Tat epitope efficiently stabilized the refolding of monomeric peptide-HLA A6801 complexes in vitro and fluorochrome-labelled, tetrameric peptide-HLA A6801 complexes stained CD8 T cells specific for this epitope directly ex vivo. |
| spellingShingle | Oxenius, A Jakobsen, B Easterbrook, P Boulter, J Tun, T Waters, A Agudelo, J Barnardo, M Phillips, R Price, D Complete mapping of a novel HLA A*6801-restricted HIV-1 Tat epitope directly with a rapid modified enzyme-linked immunospot assay. |
| title | Complete mapping of a novel HLA A*6801-restricted HIV-1 Tat epitope directly with a rapid modified enzyme-linked immunospot assay. |
| title_full | Complete mapping of a novel HLA A*6801-restricted HIV-1 Tat epitope directly with a rapid modified enzyme-linked immunospot assay. |
| title_fullStr | Complete mapping of a novel HLA A*6801-restricted HIV-1 Tat epitope directly with a rapid modified enzyme-linked immunospot assay. |
| title_full_unstemmed | Complete mapping of a novel HLA A*6801-restricted HIV-1 Tat epitope directly with a rapid modified enzyme-linked immunospot assay. |
| title_short | Complete mapping of a novel HLA A*6801-restricted HIV-1 Tat epitope directly with a rapid modified enzyme-linked immunospot assay. |
| title_sort | complete mapping of a novel hla a 6801 restricted hiv 1 tat epitope directly with a rapid modified enzyme linked immunospot assay |
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