Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro.
Fluorescence recovery after photobleaching (FRAP) is a microscopy-based technique to study the movement of fluorescent molecules inside a cell. Although initially developed to investigate intracellular mobility, FRAP can be also used to measure intercellular dynamics. This chapter describes how to p...
| Main Authors: | , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Springer
2016
|
| _version_ | 1797075307126063104 |
|---|---|
| author | Kuzma-Kuzniarska, M Yapp, C Hulley, P |
| author_facet | Kuzma-Kuzniarska, M Yapp, C Hulley, P |
| author_sort | Kuzma-Kuzniarska, M |
| collection | OXFORD |
| description | Fluorescence recovery after photobleaching (FRAP) is a microscopy-based technique to study the movement of fluorescent molecules inside a cell. Although initially developed to investigate intracellular mobility, FRAP can be also used to measure intercellular dynamics. This chapter describes how to perform FRAP experiment to study gap junctional communication in living cells. The procedures described here can be carried out with a laser-scanning confocal microscope and any in vitro cultured cells known to communicate via gap junctions. In addition, the method can be easily adjusted to measure gap junction function in 3D cell cultures as well as ex vivo tissue. |
| first_indexed | 2024-03-06T23:48:37Z |
| format | Journal article |
| id | oxford-uuid:71d40cfe-c054-47e0-8851-d1879cd9ca0c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:48:37Z |
| publishDate | 2016 |
| publisher | Springer |
| record_format | dspace |
| spelling | oxford-uuid:71d40cfe-c054-47e0-8851-d1879cd9ca0c2022-03-26T19:46:07ZUsing Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:71d40cfe-c054-47e0-8851-d1879cd9ca0cEnglishSymplectic Elements at OxfordSpringer2016Kuzma-Kuzniarska, MYapp, CHulley, PFluorescence recovery after photobleaching (FRAP) is a microscopy-based technique to study the movement of fluorescent molecules inside a cell. Although initially developed to investigate intracellular mobility, FRAP can be also used to measure intercellular dynamics. This chapter describes how to perform FRAP experiment to study gap junctional communication in living cells. The procedures described here can be carried out with a laser-scanning confocal microscope and any in vitro cultured cells known to communicate via gap junctions. In addition, the method can be easily adjusted to measure gap junction function in 3D cell cultures as well as ex vivo tissue. |
| spellingShingle | Kuzma-Kuzniarska, M Yapp, C Hulley, P Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro. |
| title | Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro. |
| title_full | Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro. |
| title_fullStr | Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro. |
| title_full_unstemmed | Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro. |
| title_short | Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro. |
| title_sort | using fluorescence recovery after photobleaching to study gap junctional communication in vitro |
| work_keys_str_mv | AT kuzmakuzniarskam usingfluorescencerecoveryafterphotobleachingtostudygapjunctionalcommunicationinvitro AT yappc usingfluorescencerecoveryafterphotobleachingtostudygapjunctionalcommunicationinvitro AT hulleyp usingfluorescencerecoveryafterphotobleachingtostudygapjunctionalcommunicationinvitro |