In situ assay for analyzing the chromatin binding of proteins in fission yeast.

An in situ technique for studying the chromatin binding of proteins in single fission yeast cells (Schizosaccharomyces pombe) is described. Cells are permeabilized by enzymatic digestion and extracted with a detergent-containing buffer. This procedure removes soluble proteins, but proteins that are...

Szczegółowa specyfikacja

Opis bibliograficzny
Główni autorzy: Kearsey, S, Brimage, L, Namdar, M, Ralph, E, Yang, X
Format: Journal article
Język:English
Wydane: 2005