A fluorimetric and amperometric study of calcium and secretion in isolated mouse pancreatic beta-cells.
We have examined the temporal relationship between intracellular Ca2+ concentration ([Ca2+]i) and secretion in single intact pancreatic beta-cells. Secretion was detected as the release of 5-hydroxytryptamine from pre-loaded beta-cells, using amperometry, and changes in [Ca2+]i were monitored by mic...
Main Authors: | Smith, P, Duchen, MR, Ashcroft, F |
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Format: | Journal article |
Language: | English |
Published: |
1995
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