Identification of VPS13C as a Galectin-12-binding protein that regulates Galectin-12 protein stability and adipogenesis
Galectin-12, a member of the galectin family of β-galactoside-binding animal lectins, is preferentially expressed in adipocytes and required for adipocyte differentiation in vitro. This protein was recently found to regulate lipolysis, whole body adiposity, and glucose homeostasis in vivo. Here we i...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
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Public Library of Science
2016
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| _version_ | 1797075428108664832 |
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| author | Yang, R Xue, H Yu, L Velayos-Baeza, A Monaco, A Liu, F |
| author_facet | Yang, R Xue, H Yu, L Velayos-Baeza, A Monaco, A Liu, F |
| author_sort | Yang, R |
| collection | OXFORD |
| description | Galectin-12, a member of the galectin family of β-galactoside-binding animal lectins, is preferentially expressed in adipocytes and required for adipocyte differentiation in vitro. This protein was recently found to regulate lipolysis, whole body adiposity, and glucose homeostasis in vivo. Here we identify VPS13C, a member of the VPS13 family of vacuolar protein sorting-associated proteins highly conserved throughout eukaryotic evolution, as a major galectin-12-binding protein. VPS13C is upregulated during adipocyte differentiation, and is required for galectin-12 protein stability. Knockdown of Vps13c markedly reduces the steady-state levels of galectin-12 by promoting its degradation through primarily the lysosomal pathway, and impairs adipocyte differentiation. Our studies also suggest that VPS13C may have a broader role in protein quality control. The regulation of galectin-12 stability by VPS13C could potentially be exploited for therapeutic intervention of obesity and related metabolic diseases. |
| first_indexed | 2024-03-06T23:50:17Z |
| format | Journal article |
| id | oxford-uuid:725ce591-36e5-4ee4-96b5-1dad73785102 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:50:17Z |
| publishDate | 2016 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:725ce591-36e5-4ee4-96b5-1dad737851022022-03-26T19:49:38ZIdentification of VPS13C as a Galectin-12-binding protein that regulates Galectin-12 protein stability and adipogenesisJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:725ce591-36e5-4ee4-96b5-1dad73785102EnglishSymplectic Elements at OxfordPublic Library of Science2016Yang, RXue, HYu, LVelayos-Baeza, AMonaco, ALiu, FGalectin-12, a member of the galectin family of β-galactoside-binding animal lectins, is preferentially expressed in adipocytes and required for adipocyte differentiation in vitro. This protein was recently found to regulate lipolysis, whole body adiposity, and glucose homeostasis in vivo. Here we identify VPS13C, a member of the VPS13 family of vacuolar protein sorting-associated proteins highly conserved throughout eukaryotic evolution, as a major galectin-12-binding protein. VPS13C is upregulated during adipocyte differentiation, and is required for galectin-12 protein stability. Knockdown of Vps13c markedly reduces the steady-state levels of galectin-12 by promoting its degradation through primarily the lysosomal pathway, and impairs adipocyte differentiation. Our studies also suggest that VPS13C may have a broader role in protein quality control. The regulation of galectin-12 stability by VPS13C could potentially be exploited for therapeutic intervention of obesity and related metabolic diseases. |
| spellingShingle | Yang, R Xue, H Yu, L Velayos-Baeza, A Monaco, A Liu, F Identification of VPS13C as a Galectin-12-binding protein that regulates Galectin-12 protein stability and adipogenesis |
| title | Identification of VPS13C as a Galectin-12-binding protein that regulates Galectin-12 protein stability and adipogenesis |
| title_full | Identification of VPS13C as a Galectin-12-binding protein that regulates Galectin-12 protein stability and adipogenesis |
| title_fullStr | Identification of VPS13C as a Galectin-12-binding protein that regulates Galectin-12 protein stability and adipogenesis |
| title_full_unstemmed | Identification of VPS13C as a Galectin-12-binding protein that regulates Galectin-12 protein stability and adipogenesis |
| title_short | Identification of VPS13C as a Galectin-12-binding protein that regulates Galectin-12 protein stability and adipogenesis |
| title_sort | identification of vps13c as a galectin 12 binding protein that regulates galectin 12 protein stability and adipogenesis |
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