Analysis of replicative intermediates of adeno-associated virus through hirt extraction and southern blotting.
Adeno-associated virus (AAV) is a small single-stranded DNA virus that requires the presence of a helper virus, such as adenovirus or herpes virus, to efficiently replicate its genome. AAV DNA is replicated by a rolling-hairpin mechanism (Ward, 2006), and during replication several DNA intermediates...
| প্রধান লেখক: | , , , , , , |
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| বিন্যাস: | Journal article |
| ভাষা: | English |
| প্রকাশিত: |
Bio-Protocol
2017
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| _version_ | 1826278940361097216 |
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| author | Bardelli, M Zarate-Perez, F Agundez, L Jolinon, N Michael Linden, R Escalante, C Henckaerts, E |
| author_facet | Bardelli, M Zarate-Perez, F Agundez, L Jolinon, N Michael Linden, R Escalante, C Henckaerts, E |
| author_sort | Bardelli, M |
| collection | OXFORD |
| description | Adeno-associated virus (AAV) is a small single-stranded DNA virus that requires the presence of a helper virus, such as adenovirus or herpes virus, to efficiently replicate its genome. AAV DNA is replicated by a rolling-hairpin mechanism (Ward, 2006), and during replication several DNA intermediates can be detected. This detailed protocol describes how to analyze the AAV DNA intermediates formed during AAV replication using a modified Hirt extract (Hirt, 1967) procedure and Southern blotting (Southern, 1975). |
| first_indexed | 2024-03-06T23:51:26Z |
| format | Journal article |
| id | oxford-uuid:72bc62c2-af70-43bf-ad9d-acef9511f3a0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:51:26Z |
| publishDate | 2017 |
| publisher | Bio-Protocol |
| record_format | dspace |
| spelling | oxford-uuid:72bc62c2-af70-43bf-ad9d-acef9511f3a02022-03-26T19:52:01ZAnalysis of replicative intermediates of adeno-associated virus through hirt extraction and southern blotting.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:72bc62c2-af70-43bf-ad9d-acef9511f3a0EnglishSymplectic Elements at OxfordBio-Protocol2017Bardelli, MZarate-Perez, FAgundez, LJolinon, NMichael Linden, REscalante, CHenckaerts, EAdeno-associated virus (AAV) is a small single-stranded DNA virus that requires the presence of a helper virus, such as adenovirus or herpes virus, to efficiently replicate its genome. AAV DNA is replicated by a rolling-hairpin mechanism (Ward, 2006), and during replication several DNA intermediates can be detected. This detailed protocol describes how to analyze the AAV DNA intermediates formed during AAV replication using a modified Hirt extract (Hirt, 1967) procedure and Southern blotting (Southern, 1975). |
| spellingShingle | Bardelli, M Zarate-Perez, F Agundez, L Jolinon, N Michael Linden, R Escalante, C Henckaerts, E Analysis of replicative intermediates of adeno-associated virus through hirt extraction and southern blotting. |
| title | Analysis of replicative intermediates of adeno-associated virus through hirt extraction and southern blotting. |
| title_full | Analysis of replicative intermediates of adeno-associated virus through hirt extraction and southern blotting. |
| title_fullStr | Analysis of replicative intermediates of adeno-associated virus through hirt extraction and southern blotting. |
| title_full_unstemmed | Analysis of replicative intermediates of adeno-associated virus through hirt extraction and southern blotting. |
| title_short | Analysis of replicative intermediates of adeno-associated virus through hirt extraction and southern blotting. |
| title_sort | analysis of replicative intermediates of adeno associated virus through hirt extraction and southern blotting |
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