Culture and differentiation of rat neural stem/progenitor cells in a three-dimensional collagen scaffold.
A stable and fast method for constructing a neural-like tissue from rat neural stem/progenitor cells (rNS/PCs) based on three-dimensional (3D) collagen gel is described. First step, the collagen-embedded rNS/PCs expanded with the medium consisting of DMEM/F12/RPMI1640 (1:1:1) supplemented with EGF a...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Journal article |
Language: | English |
Published: |
2013
|