Identification of the epidermolysis bullosa acquisita antigen by LH 7.2 monoclonal antibody: use in diagnosis.
The sera from two patients with epidermolysis bullosa acquisita were blotted against dermal extracts in comparison with the mouse monoclonal antibody LH 7.2. This antibody reacts with carboxy terminal region of type VII collagen. The epidermolysis bullosa acquisita antisera showed binding to the sam...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1989
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| _version_ | 1797075579714928640 |
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| author | Tatnall, F Whitehead, P Black, M Wojnarowska, F Leigh, I |
| author_facet | Tatnall, F Whitehead, P Black, M Wojnarowska, F Leigh, I |
| author_sort | Tatnall, F |
| collection | OXFORD |
| description | The sera from two patients with epidermolysis bullosa acquisita were blotted against dermal extracts in comparison with the mouse monoclonal antibody LH 7.2. This antibody reacts with carboxy terminal region of type VII collagen. The epidermolysis bullosa acquisita antisera showed binding to the same molecular weight protein as LH 7.2 confirming that the target antigen for epidermolysis bullosa acquisita antibodies is the carboxy terminal region of type VII collagen. This newly described collagen forms the major component of anchoring fibrils. These findings are consistent with established ultrastructural data which have shown that the epidermolysis bullosa acquisita antigen is located within and below the lamina densa. The monoclonal antibody LH 7.2 provides an internal standard for epidermolysis bullosa acquisita autoantisera activity. The use of immunoblotting of epidermolysis bullosa autoantisera in comparison with the monoclonal antibody LH 7.2 provides definitive investigation for the diagnosis of this disorder. |
| first_indexed | 2024-03-06T23:52:20Z |
| format | Journal article |
| id | oxford-uuid:7306e56c-b140-42e7-aa11-2c0eb27c2a85 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:52:20Z |
| publishDate | 1989 |
| record_format | dspace |
| spelling | oxford-uuid:7306e56c-b140-42e7-aa11-2c0eb27c2a852022-03-26T19:53:51ZIdentification of the epidermolysis bullosa acquisita antigen by LH 7.2 monoclonal antibody: use in diagnosis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7306e56c-b140-42e7-aa11-2c0eb27c2a85EnglishSymplectic Elements at Oxford1989Tatnall, FWhitehead, PBlack, MWojnarowska, FLeigh, IThe sera from two patients with epidermolysis bullosa acquisita were blotted against dermal extracts in comparison with the mouse monoclonal antibody LH 7.2. This antibody reacts with carboxy terminal region of type VII collagen. The epidermolysis bullosa acquisita antisera showed binding to the same molecular weight protein as LH 7.2 confirming that the target antigen for epidermolysis bullosa acquisita antibodies is the carboxy terminal region of type VII collagen. This newly described collagen forms the major component of anchoring fibrils. These findings are consistent with established ultrastructural data which have shown that the epidermolysis bullosa acquisita antigen is located within and below the lamina densa. The monoclonal antibody LH 7.2 provides an internal standard for epidermolysis bullosa acquisita autoantisera activity. The use of immunoblotting of epidermolysis bullosa autoantisera in comparison with the monoclonal antibody LH 7.2 provides definitive investigation for the diagnosis of this disorder. |
| spellingShingle | Tatnall, F Whitehead, P Black, M Wojnarowska, F Leigh, I Identification of the epidermolysis bullosa acquisita antigen by LH 7.2 monoclonal antibody: use in diagnosis. |
| title | Identification of the epidermolysis bullosa acquisita antigen by LH 7.2 monoclonal antibody: use in diagnosis. |
| title_full | Identification of the epidermolysis bullosa acquisita antigen by LH 7.2 monoclonal antibody: use in diagnosis. |
| title_fullStr | Identification of the epidermolysis bullosa acquisita antigen by LH 7.2 monoclonal antibody: use in diagnosis. |
| title_full_unstemmed | Identification of the epidermolysis bullosa acquisita antigen by LH 7.2 monoclonal antibody: use in diagnosis. |
| title_short | Identification of the epidermolysis bullosa acquisita antigen by LH 7.2 monoclonal antibody: use in diagnosis. |
| title_sort | identification of the epidermolysis bullosa acquisita antigen by lh 7 2 monoclonal antibody use in diagnosis |
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