The method of deriving human T-cell clones alters the proportion of IL-10-producing cells.
It is now well documented that the mode of primary stimulation in the mouse determines the ratio of Th1-, Th0- or Th2-type T cells obtained. In this paper we determine whether driving and cloning human peripheral blood mononuclear cells (PBMC) non-specifically with interleukin-2 (IL-2) and/or IL-4 a...
| Main Authors: | , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
1996
|
| _version_ | 1797075670021439488 |
|---|---|
| author | Cohen, S Webb, L Feldmann, M |
| author_facet | Cohen, S Webb, L Feldmann, M |
| author_sort | Cohen, S |
| collection | OXFORD |
| description | It is now well documented that the mode of primary stimulation in the mouse determines the ratio of Th1-, Th0- or Th2-type T cells obtained. In this paper we determine whether driving and cloning human peripheral blood mononuclear cells (PBMC) non-specifically with interleukin-2 (IL-2) and/or IL-4 and mitogen, will differentially select T cells for IL-10 production. The presence of IL-2 during culture (with or without IL-4) yielded predominantly Th1-type clones (81% of clones with IL-2 alone and 77% with IL-2 + IL-4) and a low frequency of Th0-type clones (19% of clones with IL-2 and 10% of clones with IL-2 + IL-4), whereas IL-4 alone increased the yield of the IL-4 producing Th0 (35%) clones. The proportions of IL-2-producing clones did not alter with treatment; however, the combination of IL-2 + IL-4 altered the proportions of IL-10-producing clones. 47% of IL-2-cultured cells and 51% of IL-4-cultured cells produced IL-10 respectively, whereas only 22% of clones driven with IL-2 + IL-4 produced detectable levels of IL-10. Thus in the preliminary experiments described here we have demonstrated that the cytokines used to initially drive out human T cells and maintain the T-cell growth can skew the Th1/Th2/Th0 cytokine profiles of the clones obtained from mitogen-stimulated cultures. Moreover and unexpectedly the proportion of IL-10-producing cells is altered. These results are important in interpreting analysis of the cytokine profiles of human T cells and raise questions concerning how we should derive T-cell clones for a cytokine analysis that truly reflects the in vivo situation. |
| first_indexed | 2024-03-06T23:53:31Z |
| format | Journal article |
| id | oxford-uuid:7370c8e8-f4c1-48b8-81f4-78d5969b1ae5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:53:31Z |
| publishDate | 1996 |
| record_format | dspace |
| spelling | oxford-uuid:7370c8e8-f4c1-48b8-81f4-78d5969b1ae52022-03-26T19:56:20ZThe method of deriving human T-cell clones alters the proportion of IL-10-producing cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7370c8e8-f4c1-48b8-81f4-78d5969b1ae5EnglishSymplectic Elements at Oxford1996Cohen, SWebb, LFeldmann, MIt is now well documented that the mode of primary stimulation in the mouse determines the ratio of Th1-, Th0- or Th2-type T cells obtained. In this paper we determine whether driving and cloning human peripheral blood mononuclear cells (PBMC) non-specifically with interleukin-2 (IL-2) and/or IL-4 and mitogen, will differentially select T cells for IL-10 production. The presence of IL-2 during culture (with or without IL-4) yielded predominantly Th1-type clones (81% of clones with IL-2 alone and 77% with IL-2 + IL-4) and a low frequency of Th0-type clones (19% of clones with IL-2 and 10% of clones with IL-2 + IL-4), whereas IL-4 alone increased the yield of the IL-4 producing Th0 (35%) clones. The proportions of IL-2-producing clones did not alter with treatment; however, the combination of IL-2 + IL-4 altered the proportions of IL-10-producing clones. 47% of IL-2-cultured cells and 51% of IL-4-cultured cells produced IL-10 respectively, whereas only 22% of clones driven with IL-2 + IL-4 produced detectable levels of IL-10. Thus in the preliminary experiments described here we have demonstrated that the cytokines used to initially drive out human T cells and maintain the T-cell growth can skew the Th1/Th2/Th0 cytokine profiles of the clones obtained from mitogen-stimulated cultures. Moreover and unexpectedly the proportion of IL-10-producing cells is altered. These results are important in interpreting analysis of the cytokine profiles of human T cells and raise questions concerning how we should derive T-cell clones for a cytokine analysis that truly reflects the in vivo situation. |
| spellingShingle | Cohen, S Webb, L Feldmann, M The method of deriving human T-cell clones alters the proportion of IL-10-producing cells. |
| title | The method of deriving human T-cell clones alters the proportion of IL-10-producing cells. |
| title_full | The method of deriving human T-cell clones alters the proportion of IL-10-producing cells. |
| title_fullStr | The method of deriving human T-cell clones alters the proportion of IL-10-producing cells. |
| title_full_unstemmed | The method of deriving human T-cell clones alters the proportion of IL-10-producing cells. |
| title_short | The method of deriving human T-cell clones alters the proportion of IL-10-producing cells. |
| title_sort | method of deriving human t cell clones alters the proportion of il 10 producing cells |
| work_keys_str_mv | AT cohens themethodofderivinghumantcellclonesalterstheproportionofil10producingcells AT webbl themethodofderivinghumantcellclonesalterstheproportionofil10producingcells AT feldmannm themethodofderivinghumantcellclonesalterstheproportionofil10producingcells AT cohens methodofderivinghumantcellclonesalterstheproportionofil10producingcells AT webbl methodofderivinghumantcellclonesalterstheproportionofil10producingcells AT feldmannm methodofderivinghumantcellclonesalterstheproportionofil10producingcells |