A two-dimensional DNA array: the three-layer logpile.
We describe the three-layer logpile (3LL), a two-dimensional DNA array which self-assembles from four synthetic oligonucleotides via a four-armed Holliday junction motif. It consists of three layers of helices, each running at 60 degrees to the others. DNA arrays can be used as periodic templates to...
| Hauptverfasser: | , , |
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| Format: | Journal article |
| Sprache: | English |
| Veröffentlicht: |
2009
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| _version_ | 1826279193038553088 |
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| author | Malo, J Mitchell, J Turberfield, A |
| author_facet | Malo, J Mitchell, J Turberfield, A |
| author_sort | Malo, J |
| collection | OXFORD |
| description | We describe the three-layer logpile (3LL), a two-dimensional DNA array which self-assembles from four synthetic oligonucleotides via a four-armed Holliday junction motif. It consists of three layers of helices, each running at 60 degrees to the others. DNA arrays can be used as periodic templates to create, for example, synthetic protein crystals: this array is designed to maximize structural order by ensuring that helices run continuously, without bending, through the structure. UV absorbance measurements show a rate-dependent hysteresis associated with the assembly of the 3LL. Negative-stain transmission electron microscopy (TEM) of 3LL samples shows that the arrays form extensive sheets (approximately microm(2)) and a process of iterative correlation mapping and averaging of small subsets of digitized TEM micrographs yields an averaged projection image that is consistent with a computer-generated model of the crystal. |
| first_indexed | 2024-03-06T23:55:06Z |
| format | Journal article |
| id | oxford-uuid:73f0e28c-e33b-40a8-8bea-7fac5178e74e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:55:06Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:73f0e28c-e33b-40a8-8bea-7fac5178e74e2022-03-26T19:59:42ZA two-dimensional DNA array: the three-layer logpile.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:73f0e28c-e33b-40a8-8bea-7fac5178e74eEnglishSymplectic Elements at Oxford2009Malo, JMitchell, JTurberfield, AWe describe the three-layer logpile (3LL), a two-dimensional DNA array which self-assembles from four synthetic oligonucleotides via a four-armed Holliday junction motif. It consists of three layers of helices, each running at 60 degrees to the others. DNA arrays can be used as periodic templates to create, for example, synthetic protein crystals: this array is designed to maximize structural order by ensuring that helices run continuously, without bending, through the structure. UV absorbance measurements show a rate-dependent hysteresis associated with the assembly of the 3LL. Negative-stain transmission electron microscopy (TEM) of 3LL samples shows that the arrays form extensive sheets (approximately microm(2)) and a process of iterative correlation mapping and averaging of small subsets of digitized TEM micrographs yields an averaged projection image that is consistent with a computer-generated model of the crystal. |
| spellingShingle | Malo, J Mitchell, J Turberfield, A A two-dimensional DNA array: the three-layer logpile. |
| title | A two-dimensional DNA array: the three-layer logpile. |
| title_full | A two-dimensional DNA array: the three-layer logpile. |
| title_fullStr | A two-dimensional DNA array: the three-layer logpile. |
| title_full_unstemmed | A two-dimensional DNA array: the three-layer logpile. |
| title_short | A two-dimensional DNA array: the three-layer logpile. |
| title_sort | two dimensional dna array the three layer logpile |
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