An optimised electrochemical biosensor for the label-free detection of C-reactive protein in blood
C-reactive protein (CRP) is an acute phase protein whose levels are increased in many disorders. There exists, in particular, a great deal of interest in the correlation between blood serum levels and the severity of risk for cardiovascular disease. A sensitive, label-free, non-amplified and reusabl...
| Main Authors: | , , , |
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| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
2013
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| _version_ | 1826279390892261376 |
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| author | Bryan, T Luo, X Bueno, P Davis, J |
| author_facet | Bryan, T Luo, X Bueno, P Davis, J |
| author_sort | Bryan, T |
| collection | OXFORD |
| description | C-reactive protein (CRP) is an acute phase protein whose levels are increased in many disorders. There exists, in particular, a great deal of interest in the correlation between blood serum levels and the severity of risk for cardiovascular disease. A sensitive, label-free, non-amplified and reusable electrochemical impedimetric biosensor for the detection of CRP in blood serum was developed herein based on controlled and coverage optimised antibody immobilization on standard polycrystalline gold electrodes. Charge transfer resistance changes were highly target specific, linear with log. CRP. concentration across a 0.5-50. nM range and associated with a limit of detection of 176. pM. Significantly, the detection limits are better than those of current CRP clinical methods and the assays are potentially cheap, relatively automated, reusable, multiplexed and highly portable. The generated interfaces were capable not only of comfortably quantifying CRP across a clinically relevant range of concentrations but also of doing this in whole blood serum with interfaces that were, subsequently, reusable. The importance of optimising receptor layer resistance in maximising assay sensitivity is also detailed. © 2012. |
| first_indexed | 2024-03-06T23:58:00Z |
| format | Journal article |
| id | oxford-uuid:74e75249-260c-4a50-b02e-c0cf5f7cd37f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:58:00Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:74e75249-260c-4a50-b02e-c0cf5f7cd37f2022-03-26T20:06:05ZAn optimised electrochemical biosensor for the label-free detection of C-reactive protein in bloodJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:74e75249-260c-4a50-b02e-c0cf5f7cd37fEnglishSymplectic Elements at Oxford2013Bryan, TLuo, XBueno, PDavis, JC-reactive protein (CRP) is an acute phase protein whose levels are increased in many disorders. There exists, in particular, a great deal of interest in the correlation between blood serum levels and the severity of risk for cardiovascular disease. A sensitive, label-free, non-amplified and reusable electrochemical impedimetric biosensor for the detection of CRP in blood serum was developed herein based on controlled and coverage optimised antibody immobilization on standard polycrystalline gold electrodes. Charge transfer resistance changes were highly target specific, linear with log. CRP. concentration across a 0.5-50. nM range and associated with a limit of detection of 176. pM. Significantly, the detection limits are better than those of current CRP clinical methods and the assays are potentially cheap, relatively automated, reusable, multiplexed and highly portable. The generated interfaces were capable not only of comfortably quantifying CRP across a clinically relevant range of concentrations but also of doing this in whole blood serum with interfaces that were, subsequently, reusable. The importance of optimising receptor layer resistance in maximising assay sensitivity is also detailed. © 2012. |
| spellingShingle | Bryan, T Luo, X Bueno, P Davis, J An optimised electrochemical biosensor for the label-free detection of C-reactive protein in blood |
| title | An optimised electrochemical biosensor for the label-free detection of C-reactive protein in blood |
| title_full | An optimised electrochemical biosensor for the label-free detection of C-reactive protein in blood |
| title_fullStr | An optimised electrochemical biosensor for the label-free detection of C-reactive protein in blood |
| title_full_unstemmed | An optimised electrochemical biosensor for the label-free detection of C-reactive protein in blood |
| title_short | An optimised electrochemical biosensor for the label-free detection of C-reactive protein in blood |
| title_sort | optimised electrochemical biosensor for the label free detection of c reactive protein in blood |
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