GluN2A- and GluN2B-containing pre-synaptic N -methyl- d -aspartate receptors differentially regulate action potential-evoked Ca 2+ influx via modulation of SK channels
N-methyl-d-aspartate receptors (NMDARs) play a pivotal role in synaptic plasticity. While the functional role of post-synaptic NMDARs is well established, pre-synaptic NMDAR (pre-NMDAR) function is largely unexplored. Different pre-NMDAR subunit populations are documented at synapses, suggesting tha...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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The Royal Society
2024
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| _version_ | 1826313172729987072 |
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| author | Schmidt, CC Tong, R Emptage, NJ |
| author_facet | Schmidt, CC Tong, R Emptage, NJ |
| author_sort | Schmidt, CC |
| collection | OXFORD |
| description | N-methyl-d-aspartate receptors (NMDARs) play a pivotal role in synaptic plasticity. While the functional role of post-synaptic NMDARs is well established, pre-synaptic NMDAR (pre-NMDAR) function is largely unexplored. Different pre-NMDAR subunit populations are documented at synapses, suggesting that subunit composition influences neuronal transmission. Here, we used electrophysiological recordings at Schaffer collateral-CA1 synapses partnered with Ca2+ imaging and glutamate uncaging at boutons of CA3 pyramidal neurones to reveal two populations of pre-NMDARs that contain either the GluN2A or GluN2B subunit. Activation of the GluN2B population decreases action potential-evoked Ca2+ influx via modulation of small-conductance Ca2+-activated K+ channels, while activation of the GluN2A population does the opposite. Critically, the level of functional expression of the subunits is subject to homeostatic regulation, bidirectionally affecting short-term facilitation, thus providing a capacity for a fine adjustment of information transfer. This article is part of a discussion meeting issue ‘Long-term potentiation: 50 years on’. |
| first_indexed | 2024-09-25T04:08:59Z |
| format | Journal article |
| id | oxford-uuid:75254dbb-3e3f-4035-bf06-62d6e93026d9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-09-25T04:08:59Z |
| publishDate | 2024 |
| publisher | The Royal Society |
| record_format | dspace |
| spelling | oxford-uuid:75254dbb-3e3f-4035-bf06-62d6e93026d92024-06-10T20:04:48ZGluN2A- and GluN2B-containing pre-synaptic N -methyl- d -aspartate receptors differentially regulate action potential-evoked Ca 2+ influx via modulation of SK channelsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:75254dbb-3e3f-4035-bf06-62d6e93026d9EnglishJisc Publications RouterThe Royal Society2024Schmidt, CCTong, REmptage, NJN-methyl-d-aspartate receptors (NMDARs) play a pivotal role in synaptic plasticity. While the functional role of post-synaptic NMDARs is well established, pre-synaptic NMDAR (pre-NMDAR) function is largely unexplored. Different pre-NMDAR subunit populations are documented at synapses, suggesting that subunit composition influences neuronal transmission. Here, we used electrophysiological recordings at Schaffer collateral-CA1 synapses partnered with Ca2+ imaging and glutamate uncaging at boutons of CA3 pyramidal neurones to reveal two populations of pre-NMDARs that contain either the GluN2A or GluN2B subunit. Activation of the GluN2B population decreases action potential-evoked Ca2+ influx via modulation of small-conductance Ca2+-activated K+ channels, while activation of the GluN2A population does the opposite. Critically, the level of functional expression of the subunits is subject to homeostatic regulation, bidirectionally affecting short-term facilitation, thus providing a capacity for a fine adjustment of information transfer. This article is part of a discussion meeting issue ‘Long-term potentiation: 50 years on’. |
| spellingShingle | Schmidt, CC Tong, R Emptage, NJ GluN2A- and GluN2B-containing pre-synaptic N -methyl- d -aspartate receptors differentially regulate action potential-evoked Ca 2+ influx via modulation of SK channels |
| title | GluN2A- and GluN2B-containing pre-synaptic N -methyl- d -aspartate receptors differentially regulate action potential-evoked Ca 2+ influx via modulation of SK channels |
| title_full | GluN2A- and GluN2B-containing pre-synaptic N -methyl- d -aspartate receptors differentially regulate action potential-evoked Ca 2+ influx via modulation of SK channels |
| title_fullStr | GluN2A- and GluN2B-containing pre-synaptic N -methyl- d -aspartate receptors differentially regulate action potential-evoked Ca 2+ influx via modulation of SK channels |
| title_full_unstemmed | GluN2A- and GluN2B-containing pre-synaptic N -methyl- d -aspartate receptors differentially regulate action potential-evoked Ca 2+ influx via modulation of SK channels |
| title_short | GluN2A- and GluN2B-containing pre-synaptic N -methyl- d -aspartate receptors differentially regulate action potential-evoked Ca 2+ influx via modulation of SK channels |
| title_sort | glun2a and glun2b containing pre synaptic n methyl d aspartate receptors differentially regulate action potential evoked ca 2 influx via modulation of sk channels |
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