Investigation into the mechanisms of cytoophidia assembly in drosophila melanogaster
<p>Subcellular sequestration of proteins within membrane bound compartments is widely acknowledged to be an important mode of enzymatic regulation. Recently a novel paradigm for metabolic enzyme compartmentalisation has become apparent with the identification of several proteins which are able...
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| Format: | Thesis |
| Language: | English |
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2014
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| _version_ | 1797112587377180672 |
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| author | Aughey, GN |
| author2 | Liu, JL |
| author_facet | Liu, JL Aughey, GN |
| author_sort | Aughey, GN |
| collection | OXFORD |
| description | <p>Subcellular sequestration of proteins within membrane bound compartments is widely acknowledged to be an important mode of enzymatic regulation. Recently a novel paradigm for metabolic enzyme compartmentalisation has become apparent with the identification of several proteins which are able to form filamentous structures <em>in vivo</em>. Multiple studies independently identified the essential <em>de novo</em> pyrimidine biosynthesis enzyme CTP synthetase as a major constituent of a novel filamentous structure which has been termed “the cytoophidium”. Cytoophidia have been observed to form in multiple organisms including bacteria (<em>C. crescentus</em>), yeast (<em>S. cerevisiae</em>) and fruit fly (<em>D. melanogaster</em>) as well as in human cultured cells.</p><p>In this thesis I describe the development and results of a high throughput genomescale screen to identify factors involved in cytoophidia biogenesis. Observations of tissue specific CTPS distribution lead to the identification of the well-conserved growth regulator <em>dm</em>/dMyc as an essential factor for CTPS regulation <em>in vivo</em>. These results provide new insights into the coordination of cellular growth and metabolic regulation during normal development and indicate the potential of CTPS/cytoophidia as a future therapeutic target.</p> |
| first_indexed | 2024-03-07T08:27:40Z |
| format | Thesis |
| id | oxford-uuid:7561b71c-9824-4358-8175-2b8bb4a7196e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:27:40Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | oxford-uuid:7561b71c-9824-4358-8175-2b8bb4a7196e2024-02-21T13:14:31ZInvestigation into the mechanisms of cytoophidia assembly in drosophila melanogasterThesishttp://purl.org/coar/resource_type/c_db06uuid:7561b71c-9824-4358-8175-2b8bb4a7196eCell Biology (see also Plant sciences)Life SciencesGenetics (life sciences)EnglishOxford University Research Archive - Valet2014Aughey, GNLiu, JL<p>Subcellular sequestration of proteins within membrane bound compartments is widely acknowledged to be an important mode of enzymatic regulation. Recently a novel paradigm for metabolic enzyme compartmentalisation has become apparent with the identification of several proteins which are able to form filamentous structures <em>in vivo</em>. Multiple studies independently identified the essential <em>de novo</em> pyrimidine biosynthesis enzyme CTP synthetase as a major constituent of a novel filamentous structure which has been termed “the cytoophidium”. Cytoophidia have been observed to form in multiple organisms including bacteria (<em>C. crescentus</em>), yeast (<em>S. cerevisiae</em>) and fruit fly (<em>D. melanogaster</em>) as well as in human cultured cells.</p><p>In this thesis I describe the development and results of a high throughput genomescale screen to identify factors involved in cytoophidia biogenesis. Observations of tissue specific CTPS distribution lead to the identification of the well-conserved growth regulator <em>dm</em>/dMyc as an essential factor for CTPS regulation <em>in vivo</em>. These results provide new insights into the coordination of cellular growth and metabolic regulation during normal development and indicate the potential of CTPS/cytoophidia as a future therapeutic target.</p> |
| spellingShingle | Cell Biology (see also Plant sciences) Life Sciences Genetics (life sciences) Aughey, GN Investigation into the mechanisms of cytoophidia assembly in drosophila melanogaster |
| title | Investigation into the mechanisms of cytoophidia assembly in drosophila melanogaster |
| title_full | Investigation into the mechanisms of cytoophidia assembly in drosophila melanogaster |
| title_fullStr | Investigation into the mechanisms of cytoophidia assembly in drosophila melanogaster |
| title_full_unstemmed | Investigation into the mechanisms of cytoophidia assembly in drosophila melanogaster |
| title_short | Investigation into the mechanisms of cytoophidia assembly in drosophila melanogaster |
| title_sort | investigation into the mechanisms of cytoophidia assembly in drosophila melanogaster |
| topic | Cell Biology (see also Plant sciences) Life Sciences Genetics (life sciences) |
| work_keys_str_mv | AT augheygn investigationintothemechanismsofcytoophidiaassemblyindrosophilamelanogaster |