Mutagenically separated PCR assay for rapid detection of M41L and K70R zidovudine resistance mutations in CRF01_AE (subtype E) human immunodeficiency virus type 1.
A rapid zidovudine (ZDV) resistance genotypic assay was developed based on the mutagenically separated PCR (MS-PCR) technique to detect two ZDV-resistant mutations, M41L and K70R in CRF01_AE (subtype E). Endpoint dilution analysis revealed that the newly constructed MS-PCR assay could successfully d...
Автори: | Myint, L, Ariyoshi, K, Yan, H, Frater, A, Auwanit, W, Pathipvanith, P, Yamada, K, Matsuda, M, Chiba, T, Fujita, K, McClure, M, Weber, J, Sugiura, W |
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Формат: | Journal article |
Мова: | English |
Опубліковано: |
2002
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