Dantu blood group erythrocytes form large Plasmodium falciparum rosettes less commonly
<p>Dantu erythrocytes, which express a hybrid glycophorin B/A protein, are protective against severe malaria. Recent studies have shown that Dantu impairs <em>Plasmodium falciparum</em> invasion by increasing erythrocyte membrane tension, but its effects on pathol...
| Asıl Yazarlar: | , , , , , |
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| Materyal Türü: | Journal article |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
American Society of Tropical Medicine and Hygiene
2024
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| _version_ | 1826312968163295232 |
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| author | Carlier, MSA Nyamu, W Makale, J Williams, TN Rowe, JA Kariuki, SN |
| author_facet | Carlier, MSA Nyamu, W Makale, J Williams, TN Rowe, JA Kariuki, SN |
| author_sort | Carlier, MSA |
| collection | OXFORD |
| description | <p>Dantu erythrocytes, which express a hybrid glycophorin B/A protein, are protective against severe malaria. Recent studies have shown that Dantu impairs <em>Plasmodium falciparum</em> invasion by increasing erythrocyte membrane tension, but its effects on pathological host–parasite adhesion interactions such as rosetting, the binding of uninfected erythrocytes to <em>P. falciparum</em>–infected erythrocytes, have not been investigated previously. The expression of several putative host rosetting receptors—including glycophorin A (GYPA), glycophorin C (GYPC), complement receptor 1 (CR1), and band 3, which complexes with GYPA to form the Wright<sup>b</sup> blood group antigen—are altered on Dantu erythrocytes. Here, we compare receptor expression, and rosetting at both 1 hour and 48 hours after mixing with mature trophozoite-stage Kenyan laboratory–adapted <em>P. falciparum</em> strain 11019 parasites in Dantu and non-Dantu erythrocytes. Dantu erythrocytes showed lower staining for GYPA and CR1, and greater staining for band 3, as observed previously, whereas Wright<sup>b</sup> and GYPC staining did not vary significantly. No significant between-genotype differences in rosetting were seen after 1 hour, but the percentage of large rosettes was significantly less in both Dantu heterozygous (mean, 16.4%; standard error of the mean [SEM], 3.2) and homozygous donors (mean, 15.4%; SEM, 1.4) compared with non-Dantu erythrocytes (mean, 32.9%; SEM, 7.1; one-way analysis of variance, <em>P =</em> 0.025) after 48 hours. We also found positive correlations between erythrocyte mean corpuscular volume (MCV), the percentage of large rosettes (Spearman’s r<sub>s</sub> = 0.5970, <em>P =</em> 0.0043), and mean rosette size (r<sub>s</sub> = 0.5206, <em>P =</em> 0.0155). Impaired rosetting resulting from altered erythrocyte membrane receptor expression and reduced MCV might add to the protective effect of Dantu against severe malaria.</p> |
| first_indexed | 2024-03-07T08:27:40Z |
| format | Journal article |
| id | oxford-uuid:758abf66-0926-4ac1-9b3c-8e9a7e2baac1 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-09-25T04:05:14Z |
| publishDate | 2024 |
| publisher | American Society of Tropical Medicine and Hygiene |
| record_format | dspace |
| spelling | oxford-uuid:758abf66-0926-4ac1-9b3c-8e9a7e2baac12024-05-21T07:58:26ZDantu blood group erythrocytes form large Plasmodium falciparum rosettes less commonlyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:758abf66-0926-4ac1-9b3c-8e9a7e2baac1EnglishSymplectic ElementsAmerican Society of Tropical Medicine and Hygiene2024Carlier, MSANyamu, WMakale, JWilliams, TNRowe, JAKariuki, SN<p>Dantu erythrocytes, which express a hybrid glycophorin B/A protein, are protective against severe malaria. Recent studies have shown that Dantu impairs <em>Plasmodium falciparum</em> invasion by increasing erythrocyte membrane tension, but its effects on pathological host–parasite adhesion interactions such as rosetting, the binding of uninfected erythrocytes to <em>P. falciparum</em>–infected erythrocytes, have not been investigated previously. The expression of several putative host rosetting receptors—including glycophorin A (GYPA), glycophorin C (GYPC), complement receptor 1 (CR1), and band 3, which complexes with GYPA to form the Wright<sup>b</sup> blood group antigen—are altered on Dantu erythrocytes. Here, we compare receptor expression, and rosetting at both 1 hour and 48 hours after mixing with mature trophozoite-stage Kenyan laboratory–adapted <em>P. falciparum</em> strain 11019 parasites in Dantu and non-Dantu erythrocytes. Dantu erythrocytes showed lower staining for GYPA and CR1, and greater staining for band 3, as observed previously, whereas Wright<sup>b</sup> and GYPC staining did not vary significantly. No significant between-genotype differences in rosetting were seen after 1 hour, but the percentage of large rosettes was significantly less in both Dantu heterozygous (mean, 16.4%; standard error of the mean [SEM], 3.2) and homozygous donors (mean, 15.4%; SEM, 1.4) compared with non-Dantu erythrocytes (mean, 32.9%; SEM, 7.1; one-way analysis of variance, <em>P =</em> 0.025) after 48 hours. We also found positive correlations between erythrocyte mean corpuscular volume (MCV), the percentage of large rosettes (Spearman’s r<sub>s</sub> = 0.5970, <em>P =</em> 0.0043), and mean rosette size (r<sub>s</sub> = 0.5206, <em>P =</em> 0.0155). Impaired rosetting resulting from altered erythrocyte membrane receptor expression and reduced MCV might add to the protective effect of Dantu against severe malaria.</p> |
| spellingShingle | Carlier, MSA Nyamu, W Makale, J Williams, TN Rowe, JA Kariuki, SN Dantu blood group erythrocytes form large Plasmodium falciparum rosettes less commonly |
| title | Dantu blood group erythrocytes form large Plasmodium falciparum rosettes less commonly |
| title_full | Dantu blood group erythrocytes form large Plasmodium falciparum rosettes less commonly |
| title_fullStr | Dantu blood group erythrocytes form large Plasmodium falciparum rosettes less commonly |
| title_full_unstemmed | Dantu blood group erythrocytes form large Plasmodium falciparum rosettes less commonly |
| title_short | Dantu blood group erythrocytes form large Plasmodium falciparum rosettes less commonly |
| title_sort | dantu blood group erythrocytes form large plasmodium falciparum rosettes less commonly |
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