Imaging proteins at the single-molecule level
Imaging single proteins has been a long-standing ambition for advancing various fields in natural science, as for instance structural biology, biophysics, and molecular nanotechnology. In particular, revealing the distinct conformations of an individual protein is of utmost importance. Here, we show...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
National Academy of Sciences
2017
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| _version_ | 1797076132940480512 |
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| author | Longchamp, J Rauschenbach, S Abb, S Escher, C Latychevskaia, T Kern, K Fink, H |
| author_facet | Longchamp, J Rauschenbach, S Abb, S Escher, C Latychevskaia, T Kern, K Fink, H |
| author_sort | Longchamp, J |
| collection | OXFORD |
| description | Imaging single proteins has been a long-standing ambition for advancing various fields in natural science, as for instance structural biology, biophysics, and molecular nanotechnology. In particular, revealing the distinct conformations of an individual protein is of utmost importance. Here, we show the imaging of individual proteins and protein complexes by low-energy electron holography. Samples of individual proteins and protein complexes on ultraclean freestanding graphene were prepared by soft-landing electrospray ion beam deposition, which allows chemical- and conformational-specific selection and gentle deposition. Low-energy electrons do not induce radiation damage, which enables acquiring subnanometer resolution images of individual proteins (cytochrome C and BSA) as well as of protein complexes (hemoglobin), which are not the result of an averaging process. |
| first_indexed | 2024-03-06T23:59:50Z |
| format | Journal article |
| id | oxford-uuid:758c9ea3-26a9-4656-b21e-628de7621c82 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:59:50Z |
| publishDate | 2017 |
| publisher | National Academy of Sciences |
| record_format | dspace |
| spelling | oxford-uuid:758c9ea3-26a9-4656-b21e-628de7621c822022-03-26T20:10:00ZImaging proteins at the single-molecule levelJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:758c9ea3-26a9-4656-b21e-628de7621c82EnglishSymplectic Elements at OxfordNational Academy of Sciences2017Longchamp, JRauschenbach, SAbb, SEscher, CLatychevskaia, TKern, KFink, HImaging single proteins has been a long-standing ambition for advancing various fields in natural science, as for instance structural biology, biophysics, and molecular nanotechnology. In particular, revealing the distinct conformations of an individual protein is of utmost importance. Here, we show the imaging of individual proteins and protein complexes by low-energy electron holography. Samples of individual proteins and protein complexes on ultraclean freestanding graphene were prepared by soft-landing electrospray ion beam deposition, which allows chemical- and conformational-specific selection and gentle deposition. Low-energy electrons do not induce radiation damage, which enables acquiring subnanometer resolution images of individual proteins (cytochrome C and BSA) as well as of protein complexes (hemoglobin), which are not the result of an averaging process. |
| spellingShingle | Longchamp, J Rauschenbach, S Abb, S Escher, C Latychevskaia, T Kern, K Fink, H Imaging proteins at the single-molecule level |
| title | Imaging proteins at the single-molecule level |
| title_full | Imaging proteins at the single-molecule level |
| title_fullStr | Imaging proteins at the single-molecule level |
| title_full_unstemmed | Imaging proteins at the single-molecule level |
| title_short | Imaging proteins at the single-molecule level |
| title_sort | imaging proteins at the single molecule level |
| work_keys_str_mv | AT longchampj imagingproteinsatthesinglemoleculelevel AT rauschenbachs imagingproteinsatthesinglemoleculelevel AT abbs imagingproteinsatthesinglemoleculelevel AT escherc imagingproteinsatthesinglemoleculelevel AT latychevskaiat imagingproteinsatthesinglemoleculelevel AT kernk imagingproteinsatthesinglemoleculelevel AT finkh imagingproteinsatthesinglemoleculelevel |