Durable interactions of T cells with T cell receptor stimuli in the absence of a stable immunological synapse
T cells engage in two modes of interaction with antigen-presenting surfaces: stable synapses and motile kinapses. Although it is surmised that durable interactions of T cells with antigen-presenting cells involve synapses, in situ 3D imaging cannot resolve the mode of interaction. We have establishe...
| Հիմնական հեղինակներ: | , , , , , |
|---|---|
| Ձևաչափ: | Journal article |
| Հրապարակվել է: |
Elsevier
2018
|
| _version_ | 1826279546250330112 |
|---|---|
| author | Mayya, V Judokusumo, E Abu Shah, E Wiggins, C Kam, L Dustin, M |
| author_facet | Mayya, V Judokusumo, E Abu Shah, E Wiggins, C Kam, L Dustin, M |
| author_sort | Mayya, V |
| collection | OXFORD |
| description | T cells engage in two modes of interaction with antigen-presenting surfaces: stable synapses and motile kinapses. Although it is surmised that durable interactions of T cells with antigen-presenting cells involve synapses, in situ 3D imaging cannot resolve the mode of interaction. We have established in vitro 2D platforms and quantitative metrics to determine cell-intrinsic modes of interaction when T cells are faced with spatially continuous or restricted stimulation. All major resting human T cell subsets, except memory CD8 T cells, spend more time in the kinapse mode on continuous stimulatory surfaces. Surprisingly, we did not observe any concordant relationship between the mode and durability of interaction on cell-sized stimulatory spots. Naive CD8 T cells maintain kinapses for more than 3 hr before leaving stimulatory spots, whereas their memory counterparts maintain synapses for only an hour before leaving. Thus, durable interactions do not require stable synapses. |
| first_indexed | 2024-03-07T00:00:21Z |
| format | Journal article |
| id | oxford-uuid:75b9a8e0-5f5a-41f9-80ba-6106fdde01c3 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T00:00:21Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:75b9a8e0-5f5a-41f9-80ba-6106fdde01c32022-03-26T20:11:13ZDurable interactions of T cells with T cell receptor stimuli in the absence of a stable immunological synapseJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:75b9a8e0-5f5a-41f9-80ba-6106fdde01c3Symplectic Elements at OxfordElsevier2018Mayya, VJudokusumo, EAbu Shah, EWiggins, CKam, LDustin, MT cells engage in two modes of interaction with antigen-presenting surfaces: stable synapses and motile kinapses. Although it is surmised that durable interactions of T cells with antigen-presenting cells involve synapses, in situ 3D imaging cannot resolve the mode of interaction. We have established in vitro 2D platforms and quantitative metrics to determine cell-intrinsic modes of interaction when T cells are faced with spatially continuous or restricted stimulation. All major resting human T cell subsets, except memory CD8 T cells, spend more time in the kinapse mode on continuous stimulatory surfaces. Surprisingly, we did not observe any concordant relationship between the mode and durability of interaction on cell-sized stimulatory spots. Naive CD8 T cells maintain kinapses for more than 3 hr before leaving stimulatory spots, whereas their memory counterparts maintain synapses for only an hour before leaving. Thus, durable interactions do not require stable synapses. |
| spellingShingle | Mayya, V Judokusumo, E Abu Shah, E Wiggins, C Kam, L Dustin, M Durable interactions of T cells with T cell receptor stimuli in the absence of a stable immunological synapse |
| title | Durable interactions of T cells with T cell receptor stimuli in the absence of a stable immunological synapse |
| title_full | Durable interactions of T cells with T cell receptor stimuli in the absence of a stable immunological synapse |
| title_fullStr | Durable interactions of T cells with T cell receptor stimuli in the absence of a stable immunological synapse |
| title_full_unstemmed | Durable interactions of T cells with T cell receptor stimuli in the absence of a stable immunological synapse |
| title_short | Durable interactions of T cells with T cell receptor stimuli in the absence of a stable immunological synapse |
| title_sort | durable interactions of t cells with t cell receptor stimuli in the absence of a stable immunological synapse |
| work_keys_str_mv | AT mayyav durableinteractionsoftcellswithtcellreceptorstimuliintheabsenceofastableimmunologicalsynapse AT judokusumoe durableinteractionsoftcellswithtcellreceptorstimuliintheabsenceofastableimmunologicalsynapse AT abushahe durableinteractionsoftcellswithtcellreceptorstimuliintheabsenceofastableimmunologicalsynapse AT wigginsc durableinteractionsoftcellswithtcellreceptorstimuliintheabsenceofastableimmunologicalsynapse AT kaml durableinteractionsoftcellswithtcellreceptorstimuliintheabsenceofastableimmunologicalsynapse AT dustinm durableinteractionsoftcellswithtcellreceptorstimuliintheabsenceofastableimmunologicalsynapse |