Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI
Combining histology and <i>ex vivo</i> MRI from the same mouse brain is a powerful way to study brain microstructure. Mouse brains prepared for <i>ex vivo</i> MRI are often kept in storage solution for months, potentially becoming brittle and showing reduced antigenicity. Her...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Cell Press
2023
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| _version_ | 1797111601950621696 |
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| author | Smart, A Tisca, C Huszar, IN Kor, D Ansorge, O Tachrount, M Smart, S Lerch, JP Miller, KL Martins-Bach, AB |
| author_facet | Smart, A Tisca, C Huszar, IN Kor, D Ansorge, O Tachrount, M Smart, S Lerch, JP Miller, KL Martins-Bach, AB |
| author_sort | Smart, A |
| collection | OXFORD |
| description | Combining histology and <i>ex vivo</i> MRI from the same mouse brain is a powerful way to study brain microstructure. Mouse brains prepared for <i>ex vivo</i> MRI are often kept in storage solution for months, potentially becoming brittle and showing reduced antigenicity. Here, we describe a protocol for mouse brain dissection, tissue processing, paraffin embedding, sectioning, and staining. We then detail registration of histology to <i>ex vivo</i> MRI data from the same sample and extraction of quantitative histological measurements. |
| first_indexed | 2024-03-07T08:11:10Z |
| format | Journal article |
| id | oxford-uuid:75db3889-dbf2-4628-9581-9f5e67f63997 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:11:10Z |
| publishDate | 2023 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:75db3889-dbf2-4628-9581-9f5e67f639972023-12-01T16:18:07ZProtocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRIJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:75db3889-dbf2-4628-9581-9f5e67f63997EnglishSymplectic ElementsCell Press2023Smart, ATisca, CHuszar, INKor, DAnsorge, OTachrount, MSmart, SLerch, JPMiller, KLMartins-Bach, ABCombining histology and <i>ex vivo</i> MRI from the same mouse brain is a powerful way to study brain microstructure. Mouse brains prepared for <i>ex vivo</i> MRI are often kept in storage solution for months, potentially becoming brittle and showing reduced antigenicity. Here, we describe a protocol for mouse brain dissection, tissue processing, paraffin embedding, sectioning, and staining. We then detail registration of histology to <i>ex vivo</i> MRI data from the same sample and extraction of quantitative histological measurements. |
| spellingShingle | Smart, A Tisca, C Huszar, IN Kor, D Ansorge, O Tachrount, M Smart, S Lerch, JP Miller, KL Martins-Bach, AB Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI |
| title | Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI |
| title_full | Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI |
| title_fullStr | Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI |
| title_full_unstemmed | Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI |
| title_short | Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI |
| title_sort | protocol for tissue processing and paraffin embedding of mouse brains following ex vivo mri |
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