Essential role of MCM proteins in premeiotic DNA replication.
A critical event in eukaryotic DNA replication involves association of minichromosome maintenance (MCM2-7) proteins with origins, to form prereplicative complexes (pre-RCs) that are competent for initiation. The ability of mutants defective in MCM2-7 function to complete meiosis had suggested that p...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2002
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| _version_ | 1826279578127040512 |
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| author | Lindner, K Gregán, J Montgomery, S Kearsey, S |
| author_facet | Lindner, K Gregán, J Montgomery, S Kearsey, S |
| author_sort | Lindner, K |
| collection | OXFORD |
| description | A critical event in eukaryotic DNA replication involves association of minichromosome maintenance (MCM2-7) proteins with origins, to form prereplicative complexes (pre-RCs) that are competent for initiation. The ability of mutants defective in MCM2-7 function to complete meiosis had suggested that pre-RC components could be irrelevant to premeiotic S phase. We show here that MCM2-7 proteins bind to chromatin in fission yeast cells preparing for meiosis and during premeiotic S phase in a manner suggesting they in fact are required for DNA replication in the meiotic cycle. This is confirmed by analysis of a degron mcm4 mutant, which cannot carry out premeiotic DNA replication. Later in meiosis, Mcm4 chromatin association is blocked between meiotic nuclear divisions, presumably accounting for the absence of a second round of DNA replication. Together, these results emphasize similarity between replication mechanisms in mitotic and meiotic cell cycles. |
| first_indexed | 2024-03-07T00:00:50Z |
| format | Journal article |
| id | oxford-uuid:75e49371-c3e9-4a9a-b6c4-3707dc918ae6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:00:50Z |
| publishDate | 2002 |
| record_format | dspace |
| spelling | oxford-uuid:75e49371-c3e9-4a9a-b6c4-3707dc918ae62022-03-26T20:12:16ZEssential role of MCM proteins in premeiotic DNA replication.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:75e49371-c3e9-4a9a-b6c4-3707dc918ae6EnglishSymplectic Elements at Oxford2002Lindner, KGregán, JMontgomery, SKearsey, SA critical event in eukaryotic DNA replication involves association of minichromosome maintenance (MCM2-7) proteins with origins, to form prereplicative complexes (pre-RCs) that are competent for initiation. The ability of mutants defective in MCM2-7 function to complete meiosis had suggested that pre-RC components could be irrelevant to premeiotic S phase. We show here that MCM2-7 proteins bind to chromatin in fission yeast cells preparing for meiosis and during premeiotic S phase in a manner suggesting they in fact are required for DNA replication in the meiotic cycle. This is confirmed by analysis of a degron mcm4 mutant, which cannot carry out premeiotic DNA replication. Later in meiosis, Mcm4 chromatin association is blocked between meiotic nuclear divisions, presumably accounting for the absence of a second round of DNA replication. Together, these results emphasize similarity between replication mechanisms in mitotic and meiotic cell cycles. |
| spellingShingle | Lindner, K Gregán, J Montgomery, S Kearsey, S Essential role of MCM proteins in premeiotic DNA replication. |
| title | Essential role of MCM proteins in premeiotic DNA replication. |
| title_full | Essential role of MCM proteins in premeiotic DNA replication. |
| title_fullStr | Essential role of MCM proteins in premeiotic DNA replication. |
| title_full_unstemmed | Essential role of MCM proteins in premeiotic DNA replication. |
| title_short | Essential role of MCM proteins in premeiotic DNA replication. |
| title_sort | essential role of mcm proteins in premeiotic dna replication |
| work_keys_str_mv | AT lindnerk essentialroleofmcmproteinsinpremeioticdnareplication AT greganj essentialroleofmcmproteinsinpremeioticdnareplication AT montgomerys essentialroleofmcmproteinsinpremeioticdnareplication AT kearseys essentialroleofmcmproteinsinpremeioticdnareplication |