Induction of peptide-specific primary cytotoxic T lymphocyte responses from human peripheral blood.
Various protocols were developed and compared for eliciting specific cytotoxic T lymphocyte (CTL) cell lines from the unselected human peripheral blood mononuclear cells of naive donors. Interleukin-7 and CD4+ T cells primed in vitro by keyhole limpet hemocyanin were shown to act together in the gen...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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1995
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| _version_ | 1797076246624993280 |
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| author | Plebanski, M Allsopp, C Aidoo, M Reyburn, H Hill, A |
| author_facet | Plebanski, M Allsopp, C Aidoo, M Reyburn, H Hill, A |
| author_sort | Plebanski, M |
| collection | OXFORD |
| description | Various protocols were developed and compared for eliciting specific cytotoxic T lymphocyte (CTL) cell lines from the unselected human peripheral blood mononuclear cells of naive donors. Interleukin-7 and CD4+ T cells primed in vitro by keyhole limpet hemocyanin were shown to act together in the generation of these responses. Primary responses were consistently induced with a variety of different HLA class I-binding malarial peptides. Primary CTL responses could be induced from unselected CD8+ and from CD45RA+CD8+ T cells. The CTL lines derived from these naive donors were CD8+ and demonstrated a high level of HLA class I-restricted killing for > 3 months after priming in vitro. They were also able to recognize and kill targets infected with a recombinant vaccinia virus containing the full-length antigen. In addition, this same protocol enhanced up to fourfold the levels of secondary CTL responses induced. The optimal method presented for naive cytotoxic T cell stimulation is simple, rapid and generally applicable and should provide a useful tool for both basic research and human therapy. |
| first_indexed | 2024-03-07T00:01:24Z |
| format | Journal article |
| id | oxford-uuid:7612c063-2bd3-4bc2-b729-c13d67139849 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:01:24Z |
| publishDate | 1995 |
| record_format | dspace |
| spelling | oxford-uuid:7612c063-2bd3-4bc2-b729-c13d671398492022-03-26T20:13:22ZInduction of peptide-specific primary cytotoxic T lymphocyte responses from human peripheral blood.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7612c063-2bd3-4bc2-b729-c13d67139849EnglishSymplectic Elements at Oxford1995Plebanski, MAllsopp, CAidoo, MReyburn, HHill, AVarious protocols were developed and compared for eliciting specific cytotoxic T lymphocyte (CTL) cell lines from the unselected human peripheral blood mononuclear cells of naive donors. Interleukin-7 and CD4+ T cells primed in vitro by keyhole limpet hemocyanin were shown to act together in the generation of these responses. Primary responses were consistently induced with a variety of different HLA class I-binding malarial peptides. Primary CTL responses could be induced from unselected CD8+ and from CD45RA+CD8+ T cells. The CTL lines derived from these naive donors were CD8+ and demonstrated a high level of HLA class I-restricted killing for > 3 months after priming in vitro. They were also able to recognize and kill targets infected with a recombinant vaccinia virus containing the full-length antigen. In addition, this same protocol enhanced up to fourfold the levels of secondary CTL responses induced. The optimal method presented for naive cytotoxic T cell stimulation is simple, rapid and generally applicable and should provide a useful tool for both basic research and human therapy. |
| spellingShingle | Plebanski, M Allsopp, C Aidoo, M Reyburn, H Hill, A Induction of peptide-specific primary cytotoxic T lymphocyte responses from human peripheral blood. |
| title | Induction of peptide-specific primary cytotoxic T lymphocyte responses from human peripheral blood. |
| title_full | Induction of peptide-specific primary cytotoxic T lymphocyte responses from human peripheral blood. |
| title_fullStr | Induction of peptide-specific primary cytotoxic T lymphocyte responses from human peripheral blood. |
| title_full_unstemmed | Induction of peptide-specific primary cytotoxic T lymphocyte responses from human peripheral blood. |
| title_short | Induction of peptide-specific primary cytotoxic T lymphocyte responses from human peripheral blood. |
| title_sort | induction of peptide specific primary cytotoxic t lymphocyte responses from human peripheral blood |
| work_keys_str_mv | AT plebanskim inductionofpeptidespecificprimarycytotoxictlymphocyteresponsesfromhumanperipheralblood AT allsoppc inductionofpeptidespecificprimarycytotoxictlymphocyteresponsesfromhumanperipheralblood AT aidoom inductionofpeptidespecificprimarycytotoxictlymphocyteresponsesfromhumanperipheralblood AT reyburnh inductionofpeptidespecificprimarycytotoxictlymphocyteresponsesfromhumanperipheralblood AT hilla inductionofpeptidespecificprimarycytotoxictlymphocyteresponsesfromhumanperipheralblood |