Characterization of short tandem repeats from thirty-one human telomeres.
Completion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR as...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1997
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| _version_ | 1797076406555901952 |
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| author | Rosenberg, M Hui, L Ma, J Nusbaum, H Clark, K Robinson, L Dziadzio, L Swain, P Keith, T Hudson, T Biesecker, L Flint, J |
| author_facet | Rosenberg, M Hui, L Ma, J Nusbaum, H Clark, K Robinson, L Dziadzio, L Swain, P Keith, T Hudson, T Biesecker, L Flint, J |
| author_sort | Rosenberg, M |
| collection | OXFORD |
| description | Completion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR assays were successfully designed for 58 microsatellites and mapped both genetically and on radiation hybrids (RHs) to confirm their telomeric location. Sequence analysis revealed marked variation in sequence composition, consistent with the hypothesis that even very highly GC-rich chromosome bands (the T bands) are not homogenous. The STSs that we have generated will be a necessary resource for the construction of physical maps of these complex regions of the genome. |
| first_indexed | 2024-03-07T00:03:24Z |
| format | Journal article |
| id | oxford-uuid:76bd5446-6bdb-45c7-8f63-9680d37d0c60 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:03:24Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:76bd5446-6bdb-45c7-8f63-9680d37d0c602022-03-26T20:18:16ZCharacterization of short tandem repeats from thirty-one human telomeres.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:76bd5446-6bdb-45c7-8f63-9680d37d0c60EnglishSymplectic Elements at Oxford1997Rosenberg, MHui, LMa, JNusbaum, HClark, KRobinson, LDziadzio, LSwain, PKeith, THudson, TBiesecker, LFlint, JCompletion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR assays were successfully designed for 58 microsatellites and mapped both genetically and on radiation hybrids (RHs) to confirm their telomeric location. Sequence analysis revealed marked variation in sequence composition, consistent with the hypothesis that even very highly GC-rich chromosome bands (the T bands) are not homogenous. The STSs that we have generated will be a necessary resource for the construction of physical maps of these complex regions of the genome. |
| spellingShingle | Rosenberg, M Hui, L Ma, J Nusbaum, H Clark, K Robinson, L Dziadzio, L Swain, P Keith, T Hudson, T Biesecker, L Flint, J Characterization of short tandem repeats from thirty-one human telomeres. |
| title | Characterization of short tandem repeats from thirty-one human telomeres. |
| title_full | Characterization of short tandem repeats from thirty-one human telomeres. |
| title_fullStr | Characterization of short tandem repeats from thirty-one human telomeres. |
| title_full_unstemmed | Characterization of short tandem repeats from thirty-one human telomeres. |
| title_short | Characterization of short tandem repeats from thirty-one human telomeres. |
| title_sort | characterization of short tandem repeats from thirty one human telomeres |
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