Microscope calibration using laser written fluorescence

Microscope calibration using laser written fluorescence

There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and threedimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written...

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Detalhes bibliográficos
Principais autores: Corbett, A, Shaw, M, Yacoot, A, Jefferson, A, Schermelleh, L, Wilson, T, Booth, M, Salter, P
Formato: Journal article
Publicado em: Optical Society of America 2018
Descrição
Resumo:There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and threedimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and colour plane alignment using confocal and structured illumination fluorescence microscopes.

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