Microscope calibration using laser written fluorescence
There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and threedimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Published: |
Optical Society of America
2018
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| _version_ | 1826279749012422656 |
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| author | Corbett, A Shaw, M Yacoot, A Jefferson, A Schermelleh, L Wilson, T Booth, M Salter, P |
| author_facet | Corbett, A Shaw, M Yacoot, A Jefferson, A Schermelleh, L Wilson, T Booth, M Salter, P |
| author_sort | Corbett, A |
| collection | OXFORD |
| description | There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and threedimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and colour plane alignment using confocal and structured illumination fluorescence microscopes. |
| first_indexed | 2024-03-07T00:03:25Z |
| format | Journal article |
| id | oxford-uuid:76bef4be-f1d2-4f4a-8d76-7c3d1a889fe6 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T00:03:25Z |
| publishDate | 2018 |
| publisher | Optical Society of America |
| record_format | dspace |
| spelling | oxford-uuid:76bef4be-f1d2-4f4a-8d76-7c3d1a889fe62022-03-26T20:18:20ZMicroscope calibration using laser written fluorescenceJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:76bef4be-f1d2-4f4a-8d76-7c3d1a889fe6Symplectic Elements at OxfordOptical Society of America2018Corbett, AShaw, MYacoot, AJefferson, ASchermelleh, LWilson, TBooth, MSalter, PThere is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and threedimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and colour plane alignment using confocal and structured illumination fluorescence microscopes. |
| spellingShingle | Corbett, A Shaw, M Yacoot, A Jefferson, A Schermelleh, L Wilson, T Booth, M Salter, P Microscope calibration using laser written fluorescence |
| title | Microscope calibration using laser written fluorescence |
| title_full | Microscope calibration using laser written fluorescence |
| title_fullStr | Microscope calibration using laser written fluorescence |
| title_full_unstemmed | Microscope calibration using laser written fluorescence |
| title_short | Microscope calibration using laser written fluorescence |
| title_sort | microscope calibration using laser written fluorescence |
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