Ion-specific effects on the interaction between fibronectin and negatively charged mica surfaces.
Atomic force microscopy (AFM) imaging and subsequent image analysis have been used to measure the ion-specific and ionic strength effects on the adsorption of fibronectin to mica surfaces in buffer solution. Increasing the concentration of monovalent Na(+) salt solutions is shown to cause a transiti...
| Glavni autori: | , , |
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| Format: | Journal article |
| Jezik: | English |
| Izdano: |
2010
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| _version_ | 1826279816583708672 |
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| author | Heath, MD Henderson, B Perkin, S |
| author_facet | Heath, MD Henderson, B Perkin, S |
| author_sort | Heath, MD |
| collection | OXFORD |
| description | Atomic force microscopy (AFM) imaging and subsequent image analysis have been used to measure the ion-specific and ionic strength effects on the adsorption of fibronectin to mica surfaces in buffer solution. Increasing the concentration of monovalent Na(+) salt solutions is shown to cause a transition from tightly aggregated and "string-of-beads" structures on the mica surface to well dispersed single-molecule adsorption. Studying the effect of two divalent salts, Ni(2+) and Ca(2+), reveals a dramatic enhancement of fibronectin adsorption to mica in buffer solutions containing Ni(2+), but not for Ca(2+). The origin of this ion-specific effect is discussed. |
| first_indexed | 2024-03-07T00:04:27Z |
| format | Journal article |
| id | oxford-uuid:770f6f5e-baae-4fc8-9f80-62b7731449bb |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:04:27Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:770f6f5e-baae-4fc8-9f80-62b7731449bb2022-03-26T20:20:37ZIon-specific effects on the interaction between fibronectin and negatively charged mica surfaces.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:770f6f5e-baae-4fc8-9f80-62b7731449bbEnglishSymplectic Elements at Oxford2010Heath, MDHenderson, BPerkin, SAtomic force microscopy (AFM) imaging and subsequent image analysis have been used to measure the ion-specific and ionic strength effects on the adsorption of fibronectin to mica surfaces in buffer solution. Increasing the concentration of monovalent Na(+) salt solutions is shown to cause a transition from tightly aggregated and "string-of-beads" structures on the mica surface to well dispersed single-molecule adsorption. Studying the effect of two divalent salts, Ni(2+) and Ca(2+), reveals a dramatic enhancement of fibronectin adsorption to mica in buffer solutions containing Ni(2+), but not for Ca(2+). The origin of this ion-specific effect is discussed. |
| spellingShingle | Heath, MD Henderson, B Perkin, S Ion-specific effects on the interaction between fibronectin and negatively charged mica surfaces. |
| title | Ion-specific effects on the interaction between fibronectin and negatively charged mica surfaces. |
| title_full | Ion-specific effects on the interaction between fibronectin and negatively charged mica surfaces. |
| title_fullStr | Ion-specific effects on the interaction between fibronectin and negatively charged mica surfaces. |
| title_full_unstemmed | Ion-specific effects on the interaction between fibronectin and negatively charged mica surfaces. |
| title_short | Ion-specific effects on the interaction between fibronectin and negatively charged mica surfaces. |
| title_sort | ion specific effects on the interaction between fibronectin and negatively charged mica surfaces |
| work_keys_str_mv | AT heathmd ionspecificeffectsontheinteractionbetweenfibronectinandnegativelychargedmicasurfaces AT hendersonb ionspecificeffectsontheinteractionbetweenfibronectinandnegativelychargedmicasurfaces AT perkins ionspecificeffectsontheinteractionbetweenfibronectinandnegativelychargedmicasurfaces |