Voltage sensitive dye imaging of transient neuronal assemblies in brain slices under hyperbaric conditions.
We have developed a high pressure optical cell to study large-scale transient neuronal coalitions--"assemblies" using voltage sensitive dye (VSD) fluorescence combined with fast CCD imaging of brain slices under hyperbaric conditions. The new cell has been tested at pressures up to P = 150...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2008
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| _version_ | 1797076496719806464 |
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| author | Wlodarczyk, A McMillan, P Greenfield, S |
| author_facet | Wlodarczyk, A McMillan, P Greenfield, S |
| author_sort | Wlodarczyk, A |
| collection | OXFORD |
| description | We have developed a high pressure optical cell to study large-scale transient neuronal coalitions--"assemblies" using voltage sensitive dye (VSD) fluorescence combined with fast CCD imaging of brain slices under hyperbaric conditions. The new cell has been tested at pressures up to P = 150-200 atm, corresponding to the range over which effects such as "pressure-reversal" of anaesthesia have been described previously. Brain slices were maintained in a flow of artificial cerebrospinal fluid (aCSF) and hyperbaric conditions were controlled to within +/- 0.2 atm using a back-pressure regulator placed in the pumping system. Preliminary VSD imaging experiments were carried out on rat hippocampal slices at pressures up to P approximately 50 atm. An electrode placed in the CA3 region was used to stimulate a signal along the Schaffer collateral towards CA1. First results indicate that good VSD data can be obtained that can be analysed to provide a new view on how hydrostatic pressurisation affects the dynamic propagation of neuronal assemblies. |
| first_indexed | 2024-03-07T00:04:34Z |
| format | Journal article |
| id | oxford-uuid:771cba44-c46d-4dd2-8fb2-d4ce23f857f8 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:04:34Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:771cba44-c46d-4dd2-8fb2-d4ce23f857f82022-03-26T20:21:21ZVoltage sensitive dye imaging of transient neuronal assemblies in brain slices under hyperbaric conditions.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:771cba44-c46d-4dd2-8fb2-d4ce23f857f8EnglishSymplectic Elements at Oxford2008Wlodarczyk, AMcMillan, PGreenfield, SWe have developed a high pressure optical cell to study large-scale transient neuronal coalitions--"assemblies" using voltage sensitive dye (VSD) fluorescence combined with fast CCD imaging of brain slices under hyperbaric conditions. The new cell has been tested at pressures up to P = 150-200 atm, corresponding to the range over which effects such as "pressure-reversal" of anaesthesia have been described previously. Brain slices were maintained in a flow of artificial cerebrospinal fluid (aCSF) and hyperbaric conditions were controlled to within +/- 0.2 atm using a back-pressure regulator placed in the pumping system. Preliminary VSD imaging experiments were carried out on rat hippocampal slices at pressures up to P approximately 50 atm. An electrode placed in the CA3 region was used to stimulate a signal along the Schaffer collateral towards CA1. First results indicate that good VSD data can be obtained that can be analysed to provide a new view on how hydrostatic pressurisation affects the dynamic propagation of neuronal assemblies. |
| spellingShingle | Wlodarczyk, A McMillan, P Greenfield, S Voltage sensitive dye imaging of transient neuronal assemblies in brain slices under hyperbaric conditions. |
| title | Voltage sensitive dye imaging of transient neuronal assemblies in brain slices under hyperbaric conditions. |
| title_full | Voltage sensitive dye imaging of transient neuronal assemblies in brain slices under hyperbaric conditions. |
| title_fullStr | Voltage sensitive dye imaging of transient neuronal assemblies in brain slices under hyperbaric conditions. |
| title_full_unstemmed | Voltage sensitive dye imaging of transient neuronal assemblies in brain slices under hyperbaric conditions. |
| title_short | Voltage sensitive dye imaging of transient neuronal assemblies in brain slices under hyperbaric conditions. |
| title_sort | voltage sensitive dye imaging of transient neuronal assemblies in brain slices under hyperbaric conditions |
| work_keys_str_mv | AT wlodarczyka voltagesensitivedyeimagingoftransientneuronalassembliesinbrainslicesunderhyperbaricconditions AT mcmillanp voltagesensitivedyeimagingoftransientneuronalassembliesinbrainslicesunderhyperbaricconditions AT greenfields voltagesensitivedyeimagingoftransientneuronalassembliesinbrainslicesunderhyperbaricconditions |