A practical solution for preserving single cells for RNA sequencing.

The design and implementation of single-cell experiments is often limited by their requirement for fresh starting material. We have adapted a method for histological tissue fixation using dithio-bis(succinimidyl propionate) (DSP), or Lomant's Reagent, to stabilise cell samples for single-cell t...

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Bibliographic Details
Main Authors: Attar, M, Sharma, E, Li, S, Bryer, C, Cubitt, L, Broxholme, S, Lockstone, H, Kinchen, J, Simmons, A, Piazza, P, Buck, D, Livak, K, Bowden, R
Format: Journal article
Language:English
Published: Nature Publishing Group 2018