A practical solution for preserving single cells for RNA sequencing.
The design and implementation of single-cell experiments is often limited by their requirement for fresh starting material. We have adapted a method for histological tissue fixation using dithio-bis(succinimidyl propionate) (DSP), or Lomant's Reagent, to stabilise cell samples for single-cell t...
Main Authors: | Attar, M, Sharma, E, Li, S, Bryer, C, Cubitt, L, Broxholme, S, Lockstone, H, Kinchen, J, Simmons, A, Piazza, P, Buck, D, Livak, K, Bowden, R |
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Format: | Journal article |
Language: | English |
Published: |
Nature Publishing Group
2018
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