A practical solution for preserving single cells for RNA sequencing.

A practical solution for preserving single cells for RNA sequencing.

The design and implementation of single-cell experiments is often limited by their requirement for fresh starting material. We have adapted a method for histological tissue fixation using dithio-bis(succinimidyl propionate) (DSP), or Lomant's Reagent, to stabilise cell samples for single-cell t...

Mô tả đầy đủ

Chi tiết về thư mục
Những tác giả chính:	Attar, M, Sharma, E, Li, S, Bryer, C, Cubitt, L, Broxholme, S, Lockstone, H, Kinchen, J, Simmons, A, Piazza, P, Buck, D, Livak, K, Bowden, R
Định dạng:	Journal article
Ngôn ngữ:	English
Được phát hành:	Nature Publishing Group 2018

Những quyển sách tương tự

scQCEA: a framework for annotation and quality control report of single-cell RNA-sequencing data
Bằng: Isar Nassiri, et al.
Được phát hành: (2023-07-01)

The nature and nurture of cell heterogeneity: accounting for macrophage gene-environment interactions with single-cell RNA-Seq
Bằng: Wills, Q, et al.
Được phát hành: (2017)

A modified RNA-Seq approach for whole genome sequencing of RNA viruses from faecal and blood samples.
Bằng: Batty, E, et al.
Được phát hành: (2013)

A modified RNA-Seq approach for whole genome sequencing of RNA viruses from faecal and blood samples
Bằng: Batty, E, et al.
Được phát hành: (2013)

Intestinal stromal cell types in health and inflammatory bowel disease uncovered by single-cell transcriptomics
Bằng: Kinchen, J
Được phát hành: (2017)

Demultiplexing of single-cell RNA-sequencing data using interindividual variation in gene expression
Bằng: Nassiri, I, et al.
Được phát hành: (2024)

Isolation of human fetal intestinal cells for single-cell RNA sequencing
Bằng: Fawkner-Corbett, DW, et al.
Được phát hành: (2021)

Single-Cell RNA-Sequencing Data Clustering via Locality Preserving Kernel Matrix Alignment
Bằng: Xiao Zheng, et al.
Được phát hành: (2020-01-01)

Targeted RNA sequencing enhances gene expression profiling of ultra-low input samples
Bằng: Curion, F, et al.
Được phát hành: (2020)

Isolation of human fetal intestinal cells for single-cell RNA sequencing
Bằng: David Fawkner-Corbett, et al.
Được phát hành: (2021-12-01)

A modified RNA-Seq approach for whole genome sequencing of RNA viruses from faecal and blood samples.
Bằng: Elizabeth M Batty, et al.
Được phát hành: (2013-01-01)

VIPER: variability-preserving imputation for accurate gene expression recovery in single-cell RNA sequencing studies
Bằng: Mengjie Chen, et al.
Được phát hành: (2018-11-01)

Dual RNA sequencing reveals dendritic cell reprogramming in response to typhoidal Salmonella invasion
Bằng: Aulicino, A, et al.
Được phát hành: (2022)

Dual RNA sequencing reveals dendritic cell reprogramming in response to typhoidal Salmonella invasion
Bằng: Anna Aulicino, et al.
Được phát hành: (2022-02-01)

Structural remodeling of the human colonic mesenchyme in inflammatory bowel disease
Bằng: Kinchen, J, et al.
Được phát hành: (2018)

Single-cell RNA-sequencing of the brain
Bằng: Raquel Cuevas-Diaz Duran, et al.
Được phát hành: (2017-06-01)

Zero-preserving imputation of single-cell RNA-seq data
Bằng: George C. Linderman, et al.
Được phát hành: (2022-01-01)

A practical guide to single-cell RNA-sequencing for biomedical research and clinical applications
Bằng: Ashraful Haque, et al.
Được phát hành: (2017-08-01)

Diffusion on PCA-UMAP Manifold: The Impact of Data Structure Preservation to Denoise High-Dimensional Single-Cell RNA Sequencing Data
Bằng: Padron-Manrique Cristian, et al.
Được phát hành: (2024-07-01)

Targeted single-cell RNA sequencing of transcription factors enhances the identification of cell types and trajectories
Bằng: Pokhilko, A, et al.
Được phát hành: (2021)

RNA timestamps identify the age of single molecules in RNA sequencing
Bằng: Rodriques, Samuel G, et al.
Được phát hành: (2021)

RNA timestamps identify the age of single molecules in RNA sequencing
Bằng: Rodriques, Samuel G., et al.
Được phát hành: (2022)

Improved workflows for high throughput library preparation using the transposome-based Nextera system.
Bằng: Lamble, S, et al.
Được phát hành: (2013)

The application of single-cell RNA sequencing in vaccinology
Bằng: Noé, A, et al.
Được phát hành: (2020)

Computational Methods for Single-Cell RNA Sequencing
Bằng: Hie, Brian, et al.
Được phát hành: (2021)

The Application of Single-Cell RNA Sequencing in Vaccinology
Bằng: Andrés Noé, et al.
Được phát hành: (2020-01-01)

Single-Cell RNA Sequencing in Parkinson’s Disease
Bằng: Shi-Xun Ma, et al.
Được phát hành: (2021-04-01)

Clinical Perspectives of Single-Cell RNA Sequencing
Bằng: Nayoung Kim, et al.
Được phát hành: (2021-08-01)

Single-cell RNA sequencing in orthopedic research
Bằng: Tao Wang, et al.
Được phát hành: (2023-02-01)

Single-cell RNA-sequencing in asthma research
Bằng: Weifeng Tang, et al.
Được phát hành: (2022-11-01)

Single molecular DNA/RNA sequencing with microscopy
Bằng: Masanori Kataoka, et al.
Được phát hành: (2016-02-01)

Single‐cell RNA sequencing in cancer research
Bằng: Yijie Zhang, et al.
Được phát hành: (2021-03-01)

Simultaneous single-nucleus RNA sequencing and single-nucleus ATAC sequencing of neuroblastoma cell lines
Bằng: Richard A. Guyer, et al.
Được phát hành: (2024-11-01)

Length biases in single-cell RNA sequencing of pre-mRNA
Bằng: Gennady Gorin, et al.
Được phát hành: (2023-03-01)

ve-SEQ: Robust, unbiased enrichment for streamlined detection and whole-genome sequencing of HCV and other highly diverse pathogens
Bằng: Bowden, R, et al.
Được phát hành: (2015)

Large-scale integrated genome-wide RNA sequencing, miRNA array, and genomic analyses to unravel the functionality of genome-wide association results in endometriosis
Bằng: Rahmioglu, N, et al.
Được phát hành: (2017)

Sequencing of human genomes with nanopore technology
Bằng: Bowden, R, et al.
Được phát hành: (2019)

Single cell RNA-sequencing in the upper gastrointestinal tract
Bằng: Owen, R
Được phát hành: (2018)

Extraction of total RNA from single-oocytes and single-cell mRNA sequencing of swine oocytes
Bằng: Katelyn M. Kimble, et al.
Được phát hành: (2018-02-01)

Single-cell RNA sequencing for the study of lupus nephritis
Bằng: Jill Buyon, et al.
Được phát hành: (2019-12-01)