Primary microRNA transcripts are processed co-transcriptionally.
microRNAs (miRNAs) are generated from long primary (pri-) RNA polymerase II (Pol II)-derived transcripts by two RNase III processing reactions: Drosha cleavage of nuclear pri-miRNAs and Dicer cleavage of cytoplasmic pre-miRNAs. Here we show that Drosha cleavage occurs during transcription acting on...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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| _version_ | 1797076680471216128 |
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| author | Morlando, M Ballarino, M Gromak, N Pagano, F Bozzoni, I Proudfoot, N |
| author_facet | Morlando, M Ballarino, M Gromak, N Pagano, F Bozzoni, I Proudfoot, N |
| author_sort | Morlando, M |
| collection | OXFORD |
| description | microRNAs (miRNAs) are generated from long primary (pri-) RNA polymerase II (Pol II)-derived transcripts by two RNase III processing reactions: Drosha cleavage of nuclear pri-miRNAs and Dicer cleavage of cytoplasmic pre-miRNAs. Here we show that Drosha cleavage occurs during transcription acting on both independently transcribed and intron-encoded miRNAs. We also show that both 5'-3' and 3'-5' exonucleases associate with the sites where co-transcriptional Drosha cleavage occurs, promoting intron degradation before splicing. We finally demonstrate that miRNAs can also derive from 3; flanking transcripts of Pol II genes. Our results demonstrate that multiple miRNA-containing transcripts are co-transcriptionally cleaved during their synthesis and suggest that exonucleolytic degradation from Drosha cleavage sites in pre-mRNAs may influence the splicing and maturation of numerous mRNAs. |
| first_indexed | 2024-03-07T00:07:11Z |
| format | Journal article |
| id | oxford-uuid:77ee2847-7e54-46b8-8900-9a9ffbbe18f2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:07:11Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:77ee2847-7e54-46b8-8900-9a9ffbbe18f22022-03-26T20:27:25ZPrimary microRNA transcripts are processed co-transcriptionally.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:77ee2847-7e54-46b8-8900-9a9ffbbe18f2EnglishSymplectic Elements at Oxford2008Morlando, MBallarino, MGromak, NPagano, FBozzoni, IProudfoot, NmicroRNAs (miRNAs) are generated from long primary (pri-) RNA polymerase II (Pol II)-derived transcripts by two RNase III processing reactions: Drosha cleavage of nuclear pri-miRNAs and Dicer cleavage of cytoplasmic pre-miRNAs. Here we show that Drosha cleavage occurs during transcription acting on both independently transcribed and intron-encoded miRNAs. We also show that both 5'-3' and 3'-5' exonucleases associate with the sites where co-transcriptional Drosha cleavage occurs, promoting intron degradation before splicing. We finally demonstrate that miRNAs can also derive from 3; flanking transcripts of Pol II genes. Our results demonstrate that multiple miRNA-containing transcripts are co-transcriptionally cleaved during their synthesis and suggest that exonucleolytic degradation from Drosha cleavage sites in pre-mRNAs may influence the splicing and maturation of numerous mRNAs. |
| spellingShingle | Morlando, M Ballarino, M Gromak, N Pagano, F Bozzoni, I Proudfoot, N Primary microRNA transcripts are processed co-transcriptionally. |
| title | Primary microRNA transcripts are processed co-transcriptionally. |
| title_full | Primary microRNA transcripts are processed co-transcriptionally. |
| title_fullStr | Primary microRNA transcripts are processed co-transcriptionally. |
| title_full_unstemmed | Primary microRNA transcripts are processed co-transcriptionally. |
| title_short | Primary microRNA transcripts are processed co-transcriptionally. |
| title_sort | primary microrna transcripts are processed co transcriptionally |
| work_keys_str_mv | AT morlandom primarymicrornatranscriptsareprocessedcotranscriptionally AT ballarinom primarymicrornatranscriptsareprocessedcotranscriptionally AT gromakn primarymicrornatranscriptsareprocessedcotranscriptionally AT paganof primarymicrornatranscriptsareprocessedcotranscriptionally AT bozzonii primarymicrornatranscriptsareprocessedcotranscriptionally AT proudfootn primarymicrornatranscriptsareprocessedcotranscriptionally |