Implications of the HIV-1 Rev dimer structure at 3.2 A resolution for multimeric binding to the Rev response element.
HIV-1 Rev is a small regulatory protein that mediates the nuclear export of viral mRNAs, an essential step in the HIV replication cycle. In this process Rev oligomerizes in association with a highly structured RNA motif, the Rev response element. Crystallographic studies of Rev have been hampered by...
| Main Authors: | , , , , , , , |
|---|---|
| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
2010
|
| _version_ | 1826280137338912768 |
|---|---|
| author | DiMattia, M Watts, N Stahl, S Rader, C Wingfield, P Stuart, D Steven, A Grimes, J |
| author_facet | DiMattia, M Watts, N Stahl, S Rader, C Wingfield, P Stuart, D Steven, A Grimes, J |
| author_sort | DiMattia, M |
| collection | OXFORD |
| description | HIV-1 Rev is a small regulatory protein that mediates the nuclear export of viral mRNAs, an essential step in the HIV replication cycle. In this process Rev oligomerizes in association with a highly structured RNA motif, the Rev response element. Crystallographic studies of Rev have been hampered by the protein's tendency to aggregate, but Rev has now been found to form a stable soluble equimolar complex with a specifically engineered monoclonal Fab fragment. We have determined the structure of this complex at 3.2 A resolution. It reveals a molecular dimer of Rev, bound on either side by a Fab, where the ordered portion of each Rev monomer (residues 9-65) contains two coplanar alpha-helices arranged in hairpin fashion. Subunits dimerize through overlapping of the hairpin prongs. Mating of hydrophobic patches on the outer surface of the dimer is likely to promote higher order interactions, suggesting a model for Rev oligomerization onto the viral RNA. |
| first_indexed | 2024-03-07T00:09:12Z |
| format | Journal article |
| id | oxford-uuid:789fbf4a-29d8-407b-b2ae-1304322d6f3d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:09:12Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:789fbf4a-29d8-407b-b2ae-1304322d6f3d2022-03-26T20:31:56ZImplications of the HIV-1 Rev dimer structure at 3.2 A resolution for multimeric binding to the Rev response element.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:789fbf4a-29d8-407b-b2ae-1304322d6f3dEnglishSymplectic Elements at Oxford2010DiMattia, MWatts, NStahl, SRader, CWingfield, PStuart, DSteven, AGrimes, JHIV-1 Rev is a small regulatory protein that mediates the nuclear export of viral mRNAs, an essential step in the HIV replication cycle. In this process Rev oligomerizes in association with a highly structured RNA motif, the Rev response element. Crystallographic studies of Rev have been hampered by the protein's tendency to aggregate, but Rev has now been found to form a stable soluble equimolar complex with a specifically engineered monoclonal Fab fragment. We have determined the structure of this complex at 3.2 A resolution. It reveals a molecular dimer of Rev, bound on either side by a Fab, where the ordered portion of each Rev monomer (residues 9-65) contains two coplanar alpha-helices arranged in hairpin fashion. Subunits dimerize through overlapping of the hairpin prongs. Mating of hydrophobic patches on the outer surface of the dimer is likely to promote higher order interactions, suggesting a model for Rev oligomerization onto the viral RNA. |
| spellingShingle | DiMattia, M Watts, N Stahl, S Rader, C Wingfield, P Stuart, D Steven, A Grimes, J Implications of the HIV-1 Rev dimer structure at 3.2 A resolution for multimeric binding to the Rev response element. |
| title | Implications of the HIV-1 Rev dimer structure at 3.2 A resolution for multimeric binding to the Rev response element. |
| title_full | Implications of the HIV-1 Rev dimer structure at 3.2 A resolution for multimeric binding to the Rev response element. |
| title_fullStr | Implications of the HIV-1 Rev dimer structure at 3.2 A resolution for multimeric binding to the Rev response element. |
| title_full_unstemmed | Implications of the HIV-1 Rev dimer structure at 3.2 A resolution for multimeric binding to the Rev response element. |
| title_short | Implications of the HIV-1 Rev dimer structure at 3.2 A resolution for multimeric binding to the Rev response element. |
| title_sort | implications of the hiv 1 rev dimer structure at 3 2 a resolution for multimeric binding to the rev response element |
| work_keys_str_mv | AT dimattiam implicationsofthehiv1revdimerstructureat32aresolutionformultimericbindingtotherevresponseelement AT wattsn implicationsofthehiv1revdimerstructureat32aresolutionformultimericbindingtotherevresponseelement AT stahls implicationsofthehiv1revdimerstructureat32aresolutionformultimericbindingtotherevresponseelement AT raderc implicationsofthehiv1revdimerstructureat32aresolutionformultimericbindingtotherevresponseelement AT wingfieldp implicationsofthehiv1revdimerstructureat32aresolutionformultimericbindingtotherevresponseelement AT stuartd implicationsofthehiv1revdimerstructureat32aresolutionformultimericbindingtotherevresponseelement AT stevena implicationsofthehiv1revdimerstructureat32aresolutionformultimericbindingtotherevresponseelement AT grimesj implicationsofthehiv1revdimerstructureat32aresolutionformultimericbindingtotherevresponseelement |